Mass Culturing of Ditylenchus dipsaci to Yield Large Quantities of Inoculum |
| |
| Authors: | L R Faulkner D B Bower D W Evans J H Elgin Jr |
| |
| Abstract: | Methods are described for rearing large quantities of Ditylenchus dipsaci on alfalfa tissues. Nematodes and alfalfa seed were disinfected and nematodes were reared in quantities sufficient to provide a continuous supply of inoculum for our alfalfa-breeding program. Nematodes reproduced best in darkness at 20-25 C. Cultures reached maximum numbers in 3-6 wk. |
| |
| Keywords: | tissue culture alfalfa stem nematode Medicago sativa |
|
