Expression,intracellular targeting and purification of HIV Nef variants in tobacco cells |
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Authors: | Carla Marusic James Nuttall Giampaolo Buriani Chiara Lico Raffaele Lombardi Selene Baschieri Eugenio Benvenuto Lorenzo Frigerio |
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Institution: | (1) ENEA-BIOTEC Sezione Genetica e Genomica Vegetale, C.R. Casaccia, 00060 Rome, Italy;(2) Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK |
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Abstract: | Background Plants may represent excellent alternatives to classical heterologous protein expression systems, especially for the production
of biopharmaceuticals and vaccine components. Modern vaccines are becoming increasingly complex, with the incorporation of
multiple antigens. Approaches towards developing an HIV vaccine appear to confirm this, with a combination of candidate antigens.
Among these, HIV-Nef is considered a promising target for vaccine development because immune responses directed against this
viral protein could help to control the initial steps of viral infection and to reduce viral loads and spreading. Two isoforms
of Nef protein can be found in cells: a full-length N-terminal myristoylated form (p27, 27 kDa) and a truncated form (p25,
25 kDa). Here we report the expression and purification of HIV Nef from transgenic tobacco. |
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