Effect of DNA fragment size on transformation frequencies in tobacco (Nicotiana tabacum) and maize (Zea mays) |
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Authors: | Geraldine H Fleming Catherine M Kramer Trang Le and Raymond D Shillito |
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Institution: | Agricultural Biotechnology Research Unit, CIBA Corporation, 3054 Cornwallis Road, Research Triangle Park, NC 27709, USA |
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Abstract: | During eukaryotic cell transformation, the transforming DNA must enter the host cell, traverse the cytoplasm and enter the nucleus before becoming stably integrated into the genome. The limiting step for plant protoplast transformation may lie at the cell membrane, the nuclear membrane, or at the integration step. We show here that the size of the DNA fragment containing the selectable marker used to monitor transformation can directly affect the efficiency of stable transformation. In both tobacco and maize protoplasts, the smallest DNA fragments gave the highest stable transformation frequencies. |
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Keywords: | Protoplasts Nicotiana tabacum Zea mays Stable transformation Transient expression DNA fragment size |
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