| 6-BA和氨基酸对黄瓜子叶离体培养成花的影响 |
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| 引用本文： | 周俊辉,周家容,林毕成,丁谨.6-BA和氨基酸对黄瓜子叶离体培养成花的影响[J].植物生理学通讯,2004,40(2):171-174. |
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| 作者姓名： | 周俊辉 周家容 林毕成 丁谨 |
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| 作者单位： | 1. 仲恺农业技术学院园艺系,广州,510225
2. 仲恺农业技术学院基础部,广州,510225 |
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| 基金项目： | 教育部新世纪高等教育教学改革工程项目 |
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| 摘 要： | 在基本培养基的筛选中,1/2MS培养基中附加0.10 mg·L-16-BA能显著提高离体黄瓜子叶的开花率,White培养基中附加2.00mg·L-1的KT下开花率也有明显提高,但植株矮小、瘦弱,整株变黄,甚至透明化,长势极差.浓度在一定范围的6-BA(0.01～0.50mg·L-1)对黄瓜子叶开花率影响不大,但在高浓度(0.50 mg·L-1)下,植株生长势差,开花迟,低浓度(0.01 mg·L-1)下,生长势较好.相同浓度的L-丙氨酸和L-酪氨酸均明显促进黄瓜子叶开花,且开花早;而甘氨酸对黄瓜子叶开花则有一定的抑制,开花率低.
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| 关 键 词： | 黄瓜 子叶 离体开花 基本培养基 氨基酸 |
| 修稿时间： | 2003年6月16日 |
Influences of 6-BA and Amino Acids on Flower Formation from Cotyledons of Cucumber in vitro |
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| ZHOU Jun-Hui,ZHOU Jia-Rong,LIN Bi-Cheng,DIN Jin.Influences of 6-BA and Amino Acids on Flower Formation from Cotyledons of Cucumber in vitro[J].Plant Physiology Communications,2004,40(2):171-174. |
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| Authors: | ZHOU Jun-Hui ZHOU Jia-Rong LIN Bi-Cheng DIN Jin |
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| Institution: | ZHOU Jun-Hui1,*,ZHOU Jia-Rong2,LIN Bi-Cheng1,DIN Jin11Department of Horticulture,2Basic Department,Zhongkai Agrotechnical College,Guangzhou 510225 |
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| Abstract: | Cotyledon explants without embryonic axes from Yuexiu No.1 cucumber were cultured on differ-ent media. The results indicated that, the flowering rate was obviously increased by 1/2 MS medium supple-mented with 0.10 mg.L-1 6-BA. Although the higher flowering rate was got by White medium supplementedwith 2.00 mg.L-1 KT but the plant was small, thin and etiolation. There was not obvious differences in flowerrate on 1/2MS medium containing 0.01, 0.05, 0.10 and 0.50 mg.L-1 6-BA. The plant grew well at a lowconcentration of 6-BA but at a high concentration grew badly. The flowering rate was improved obviouslyby 1/2 MS medium supplemented with 2.00 mg.L-1 L-alanine and 2.00 mg.L-1 L-tyrosine respectively, whileglycine presented inhibition, the lower flowering was recorded. |
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| Keywords: | 6-BA |
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