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外源一氧化氮供体硝普钠对干旱胁迫下小麦幼苗叶中ATP酶活性和膜脂过氧化的影响
引用本文:李慧,;赵文才,;赵会杰,;韩燕来,;谭金芳.外源一氧化氮供体硝普钠对干旱胁迫下小麦幼苗叶中ATP酶活性和膜脂过氧化的影响[J].植物生理学通讯,2009(5):455-458.
作者姓名:李慧  ;赵文才  ;赵会杰  ;韩燕来  ;谭金芳
作者单位:[1]河南农业大学 资源环境学院,郑州450002; [2]河南农业大学 生命科学学院,郑州450002
基金项目:国际植物营养研究所(IPNI)项目(30200108)、“十一五”国家科技支撑计划(30200166)和河南省农业科技成果转化基金项目(05360000006).
摘    要:研究外源一氧化氮(NO)供体硝普钠(sNP)对干旱胁迫下小麦幼苗叶片ATP酶活性和膜脂过氧化影响的结果表明,15%聚乙二醇.6000(PEG-6000)模拟的干旱胁迫下小麦幼苗叶中H^+-ATP酶和Ca^2+.ATP酶活性显著升高后迅速下降,硫代巴比妥酸反应产物(TBARs)和质量膜透性增加;0.1mm01.L^-1 SNP可提高干旱胁迫下小麦幼苗叶中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(cAT)活性,降低超氧阴离子(O2^-)和过氧化氢(H2O2)水平,缓解膜脂过氧化,稳定生物膜的结构和功能,H^+-ATP酶和Ca^2+-ATP酶也可以保持更高的活性。

关 键 词:一氧化氮  干旱胁迫  小麦幼苗叶片  ATP酶活性  膜脂过氧化

Effects of Exogenous Nitric Oxide Donor Sodium Nitroprusside on ATPase Activity and Membrane Lipid Peroxidation in Wheat (Triticum aestivum L. cv. ‘Luohan 6') Seedling Leaves under Drought Stress
Institution:LI Hui, ZHAO Wen-Cai, ZHAO Hui-Jie, HAN Yan-Lai, TAN Jin-Fang(1.College of Environmental and Resource Sciences, 2. College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China)
Abstract:The effects of nitric oxide (NO) donor sodium nitroprusside (SNP) on ATPase activity and membrane lipid peroxidation in the wheat (Triticum aestivum cv. ‘Luohan 6') seedling leaves under drought stress were investigated. Results showed that the 15% PEG-6000 treatment apparently enhanced the H^+-ATPase and Ca^2+-ATPase activities before period of deterioration, accelerated the accumulation of thiobarbituric acid reac- tive substances (TBARS) and membrane permeability in wheat seedling leaves under drought stress. 0.1 mmol.L^-1 SNP could enhance the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), lower O2^- production rate, H2O2 content and membrane lipid peroxidation, stabilize the structure and function of biomembranes, increase the activities H^+-ATPase and Ca^2+-ATPase.
Keywords:nitric oxide  drought stress  wheat seedling leaves  ATPase activity  membrane lipid peroxidation
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