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受烤烟花叶病病毒侵染后叶中保护酶活性和超微结构的变化
引用本文:刘国顺,;张彩霞,;王婵娟,;王新中,;刘清华,;李宽伟.受烤烟花叶病病毒侵染后叶中保护酶活性和超微结构的变化[J].植物生理学通讯,2009(5):459-463.
作者姓名:刘国顺  ;张彩霞  ;王婵娟  ;王新中  ;刘清华  ;李宽伟
作者单位:[1]河南农业大学国家烟草栽培生理生化研究基地,郑州450002; [2]河南省平顶山市烟草公司,河南平项山467000; [3]河南省郏县烟草公司,河南郏县467100
基金项目:国家烟草专卖局重点研究项目(TCKL06001).
摘    要:研究受侵染程度不同的烤烟花叶病烟株叶中保护酶活性和超微结构的结果表明,病毒伤害烟株的原因之一是不能及时清除活性氧,导致活性氧和丙二醛积累,引起质膜伤害,最终导致超微结构破坏,烟株生长受抑制,生物量积累下降。据此认为,烟草花叶病防治,除了防除病源外,还应注意超微结构的保护和恢复。

关 键 词:烤烟  花叶病  保护酶活性  超微结构

Changes in Protective Enzyme Activities and Ultrastructures of Flue-cured Tobacco (Nicotiana tabacum L.) Infected with Tobacco Mosaic Virus
Institution:LIU Guo-Shun, ZHANG Cai-Xia, WANG Chan-Juan, WANG Xin-Zhong, LIU Qing-Hua, LI Kuan-Wei3 (1.National Tobacco Cultivation and Physiological and Biochemical Research Center, Henan Agricultural University, Zhengzhou 450002, China; 2.Pingdingshan Tobacco Company of Henan Province, Pingdingshan, Henan 467000, China; 3.Jiaxian Tobacco Company of Henan Province, Jiaxian, Henan 467100, China)
Abstract:Protective enzyme activities and ultrastructural alternation of flue-cured tobacco (Nicotiana tabacum) infected with tobacco mosaic virus (TMV) were studied. The results showed that one of the reasons of the injury caused by virus was that the reactive oxygen species could not be cleared timely, which induced the accumulation of reactive oxygen species and malondialdehyde (MDA), further leading to membrane injury. Ultimately, ultrastructures were destructed, the growth was inhibited, and the biomass accumulation declined. All these results indicated that it is key important to protect and recover the ultrastructure as well as eliminate the disease source for tobacco mosaic disease prevention.
Keywords:flue-cured tobacco  tobacco mosaic virus  protective enzyme activities  ultrastructures
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