| 甘薯EST资源的SSR信息分析 |
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| 引用本文： | 黄立飞,房伯平,陈景益,张雄坚,罗忠霞.甘薯EST资源的SSR信息分析[J].植物生理学通讯,2009(1):23-27. |
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| 作者姓名： | 黄立飞 房伯平 陈景益 张雄坚 罗忠霞 |
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| 作者单位： | 广东省农业科学院作物研究所,广州510640 |
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| 基金项目： | 国家高技术研究发展计划（“863”计划）（2006AA100107）、国家科技基础条件平台项目（2005DKA2100205）、广东省自然科学基金项目（8151064001000033）、广东省科技计划项目（2008B020200003）. |
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| 摘 要： | 从NCBI公共数据库下载获得22371条甘薯EST序列，去除低质量的和冗余的序列后，得到总长为5．09×10^3kb的9204条唯一序列。从这些序列中搜索到总共436个SSR位点，平均相距11．68kb出现一个SSR。这些SSR的出现频率和平均长度分别为4．4％和24．28bp。在2-6bp的重复基元中，六核苷酸重复基元出现频率最（30．96％），其次是三核苷酸重复基元（29．59％）和二核苷酸重复基元（24．54％）。出现最多的重复基元是AG／CT（16．28％），其次是AAG／CTT（11．01％）。
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| 关 键 词： | 甘薯 EST—SSR 基元 |
Analysis of SSR Information in EST Resource of Sweet Potato [lpomoea batatas （L.） Lam] |
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| HUANG Li-Fei,FANG Bo-Ping,CHEN Jing-Yi,ZHANG Xiong-Jian,LUO Zhong-Xia.Analysis of SSR Information in EST Resource of Sweet Potato [lpomoea batatas （L.） Lam][J].Plant Physiology Communications,2009(1):23-27. |
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| Authors: | HUANG Li-Fei FANG Bo-Ping CHEN Jing-Yi ZHANG Xiong-Jian LUO Zhong-Xia |
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| Institution: | ( Crop Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China) |
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| Abstract: | In this study, 22 371 ESTs of sweet potato （Ipomoea batatas） in the database of NCBI were downloaded and some redundants or with low qualities were removed, finally 9 204 unique sequences with total length about 5.09× 1063 kb were obtained. In total, 436 SSRs （4.4%） containing unique sequences were identified. The overall average length of SSRs was 24.28 bp. This was equivalent to 1 SSR per 11.68 kb EST sequence. About 30.96% of the SSRs was hexanucleotides, 29.59% was trinucleotides, 24.54% was dinucleotides, and the remaining 14.91% consisted of tetra- and pentanucleotides. Among the identified SSRs, AG/CT was the most frequent （16.28%） followed by AAG/CTT at 11.01%. |
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| Keywords: | sweet potato EST-SSR motifs |
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