| 大豆重组自交系群体（ZKS-HX）遗传图谱的构建和形态标记的定位 |
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| 引用本文： | 吕祝章,;丁立孝,;田纪春,;常汝镇,;邱丽娟.大豆重组自交系群体（ZKS-HX）遗传图谱的构建和形态标记的定位[J].植物生理学通讯,2009(4):345-350. |
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| 作者姓名： | 吕祝章 ;丁立孝 ;田纪春 ;常汝镇 ;邱丽娟 |
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| 作者单位： | [1]日照职业技术学院,山东日照276826; [2]山东农业大学农学院,山东泰安271018; [3]中国农业科学院作物科学研究所,国家农作物基因资源与遗传改良重大科学工程,农业部作物种质资源与生物技术重点开放实验室,北京100081 |
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| 基金项目： | 国家自然科学基金（30490250）. |
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| 摘 要： | 以大豆品种‘合丰25’为母本，半野生大豆‘新民6号’为父本杂交得到的F2-9代122个重组自交系为试验材料，构建了含有124个SSR标记、1个EST标记、3个形态学标记的大豆遗传图谱。此图谱覆盖的基因组长度为2348．3cM．标记间平均距离为18．3cM。每个连锁群长度范围为15．1～195．9cM之间，标记数范围2—10个。本文将控制茸毛色（Pb）基因定位于LG06-C2连锁群上，与Sat_40x2的遗传距离为39．6cM；控制叶耳g（Le）、花色（4W，）基因定位于LG12-F连锁群上，它们之间的遗传距离为9．9cM，与两边的Satt348、Sat_240标记遗传距离分别为13．3cM和10．5cM。
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| 关 键 词： | 大豆 重组自交系 遗传图谱 SSR EST |
A Genetic Map of Soybean Using a Recombinant Inbred Line Population （ZKS- HX） and Mapping of Morphological Markers |
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| Institution: | LU Zhu-Zhang, DING Li-Xiao, TIAN Ji-Chun, CHANG Ru-Zhen, QIU Li-Juan(1,Rizhao Polytechnic College, Rizhao, Shandong 276826, China; 2College of Agronomy, Shandong Agricultural University, Taian, Shandong 271018, China; 3Institute of Crop Sciences, National Key Facility of Crop Gene Resources and Genetic Improvement, Key Laboratory of Crop Germplasm ＆ Biotechnology (MOA), Chinese Academy of Agricultural Sciences, Beijing 100081, China) |
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| Abstract: | A Fz：9 RIL （recombinant inbred line） population with 122 lines was derived from a cross of ＇Hefeng 25＇ as a female parent and ＇Xinmin 6＇ as a male parent. A new soybean molecular genetic map was constructed by using Mapmaker. The new soybean genetic map covered 2 348.3 cM, and the average distance among markers was 18.3 cM. The length of linkage group varied from 15.1 cM to 195.9 cM, and the markers on each linkage group varied from 2 to 10. 124 SSRs, 1 EST and 3 morphology markers were located in this map. A pubescence color （Pb） gene was located on LG06-C2 linkage group, 39.6 cM far from the marker of Sat_402. The auricle color gene （Le） and flower color gene （W） were located on LG12-F linkage group, the distance between Le and W was 9.9 cM, the distances between the two genes and the markers of Satt348 and Sat_240 were 13.3 cM and 10.5 cM, respectively. |
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| Keywords: | soybean RIL genetic map SSR EST |
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