一个新的茶树黄酮醇合酶基因的克隆和表达分析

Cs—COR113（GenBank登录号：FE942098）为一受冷诱导的茶树黄酮醇合酶基因的cDNA片段，采用RACE技术克隆了这一基因的全长cDNA，命名为CsFLS（GenBank登录号：FJ577509）。CsFLScDNA序列全长为1303bp，5＇-UTR和3’-UTR分别长91bp和175bp，包含一个编码336个氨基酸的完整开放阅读框。序列分析显示，CsFLS与烟草、矮牵牛、欧芹、拟南芥的黄酮醇合酶的同源性分别为74％、75％、75％和63％，含有2-酮戊二酸依赖性双加氧酶家族2个保守的基序，以及与黄酮醇合酶正确折叠有关的2个保守的甘氨酸残基。CsFLS的表达受低温诱导，但不受ABA诱导。

Cloning and Expression Analysis of a Flavonol Synthase Gene from Camellia sinensis

Cs-COR113 is the cDNA fragment of a cold-induced flavonol synthase （FLS） gene of tea （Camellia sinensis L.）. The full-length cDNA, which was cloned using RACE （rapid amplification of cDNA ends） strategy （designated as CsFLS, GenBank accession number： FJ577509）, was 1 303 bp in length with a 91-bp 5＇-UTR and a 175-bp 3＇-UTR, containing an open reading frame of 336 amino acids. CsFLS protein had homology to other flavonol synthase from Nicotiana tabacum, Petunia hybrida, Petroselinum crispum, and Arabidopsis thaliana, showing 74%, 75%, 75%, and 63% in amino acid identity, respectively. CsFLS contained two conserved motifs of the 2-oxoglutarate-dependent dioxygenases and two glycine residues required for proper folding of the FLS polypeptide. The expression of CsFLS was induced by low temperature, but not by ABA.