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亚麻胚性细胞悬浮培养体系的建立和影响因素
引用本文:苗书魁,;姜丽,;计巧灵,;李文婷,;热西旦·尼格买提.亚麻胚性细胞悬浮培养体系的建立和影响因素[J].植物生理学通讯,2009(12):1181-1185.
作者姓名:苗书魁  ;姜丽  ;计巧灵  ;李文婷  ;热西旦·尼格买提
作者单位:[1]新疆大学生命科学与技术学院,乌鲁木齐830046; [2]新疆轻工职业技术学院食品工程系,乌鲁木齐830021
基金项目:国家自然科学基金(30660095)和新疆教育厅重点科研项目(XJEDU2006108).
摘    要:亚麻品种‘双亚五号’的胚性愈伤组织诱导最佳培养基为MB(MS无机盐加B5维生素)+1.0mg·L^-12,4-D;细胞初始悬浮培养的最佳培养基为MB+0.2mg·L-16-BA;细胞继代悬浮培养的最佳培养基为改良的MB(NH4NO3减半,KNO3加倍)+0.02mg·L^-12,4-D+0.2mg·L^-16-BA;适宜的蔗糖量为50g·L^-1;适宜的继代接种量为1.0~1.5g·(25mL)^-1;继代间隔为5~7d。

关 键 词:亚麻  胚性愈伤组织  悬浮细胞系

Establishment and Influence Factors of Embryogenic Cell Suspension Lines of Linum usitatissimum L. in vitro
Institution:MIAO Shu-Kui, JIANG Li, JI Qiao-Ling, LI Wen-Ting, Rexidan·Nigemaiti ( 1College of Life Science and Technology, Xinjiang University, Urumqi 830046, China; 2Food Engineering Department, Xinjiang Institute of Light Industry Technology, Urumqi 830021, China)
Abstract:The optimum induction medium for the embryogenic callus of Linum usitatissimum 'Shuangya No. 5' was MB (MS inorganic salts supplemented with B5 vitamins)+ 1.0 mg·L^-1 2,4-D. The optimum medium for initial culture of suspension cells was liquid MB supplemented with 0.2 mg·L^-1 6-BA. The suspension cells were cultured on modified MB liquid medium (NH4NO3 halved, KNO3 doubled) supplemented with 0.02 mg·L^-1 2,4- D+0.2 mg·L^-16-BA for subculturing. The suitable sucrose concentration was 50 g·L^-1. The optimal inoculative densities were 1.0-1.5 g·(25 mL)^-1, and the subculture period was 5-7 d.
Keywords:Linum usitatissimum  embryogenic callus  cell suspension lines
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