| 化学诱变剂和^60Co处理对大麦小孢子低氮胁迫培养的影响 |
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| 引用本文： | 陆瑞菊,陈志伟,何婷,徐红卫,杜志钊,高润红,王亦菲,邹磊,郭桂梅,卜姝明,黄亦辰,刘成洪,黄剑华.化学诱变剂和^60Co处理对大麦小孢子低氮胁迫培养的影响[J].植物生理学通讯,2013(12):1442-1446. |
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| 作者姓名： | 陆瑞菊 陈志伟 何婷 徐红卫 杜志钊 高润红 王亦菲 邹磊 郭桂梅 卜姝明 黄亦辰 刘成洪 黄剑华 |
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| 作者单位： | [1]上海市农业科学院生物技术研究所,上海201106 [2]上海市农业遗传育种重点实验室,上海201106 [3]上海海洋大学水产与生命学院,上海201306 |
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| 基金项目： | 大麦青稞产业技术体系（CARS-05）、上海市种业发展项目[沪农科种字（2012）第7号]、上海市科委基础重点项目（12JC1407800）. |
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| 摘 要： | 以大麦品种‘花30’为材料，用不同剂量化学诱变剂（EMs和PYM）处理大麦游离小孢子，不同剂量^60Co辐照处理大麦离体穗和干种子，比较其对在低氮胁迫下小孢子培养诱导愈伤组织产量和绿苗分化数量的影响。结果表明，EMS处理离体小孢子和^60Co辐照干种子明显比PYM处理小孢子和^60Co辐照离体穗的培养效果好。
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| 关 键 词： | 大麦 小孢子培养 诱变 |
Effects of Chemical Mutagen and 60Co Treatments on Microspore Culture of Barley under Low-Nitrogen Stress |
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| LU Rui-Ju,＇,CHEN Zhi-Wei,＇,HE Tingla,XU Hong-Wei,＇,DU Zhi-Zhao,＇,GAO Run-Hong,＇,WANG Yi-Fei,＇,ZOU Lei,＇,GUO Gui-Mei,＇,BU Shu-Ming,HUANG Yi-Chen,LIU Cheng-Hong,＇,HUANG Jian-Hua.Effects of Chemical Mutagen and 60Co Treatments on Microspore Culture of Barley under Low-Nitrogen Stress[J].Plant Physiology Communications,2013(12):1442-1446. |
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| Authors: | LU Rui-Ju ＇ CHEN Zhi-Wei ＇ HE Tingla XU Hong-Wei ＇ DU Zhi-Zhao ＇ GAO Run-Hong ＇ WANG Yi-Fei ＇ ZOU Lei ＇ GUO Gui-Mei ＇ BU Shu-Ming HUANG Yi-Chen LIU Cheng-Hong ＇ HUANG Jian-Hua |
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| Institution: | 1Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 2Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201106, China; 3College of Fishery and Life Science, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | To investigate the effects of chemical mutagens and 60Co on callus yield and green plantlet production through microspore culture under low-nitrogen stress, we used the barley （Hordeum vulgare） variety ＇Hua 30＇ as the tested material, used two chemical mutagens, ethylmethylsulfone （EMS） and pingyangmycin （PYM）, with different concentrations to treat the microspores, and used 60C0 with different doses to treat young spikes and seeds respectively. The results showed that EMS treatment of microspores or 60Co treatment of seeds were much better than PYM treatment of microspores or 60Co treatment of young spikes for callus induction and green plantlet differentiation. |
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| Keywords: | Hordeum vulgare microspore culture mutation |
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