| 珍稀药用植物红根草野生及离体快繁群体的遗传多样性 |
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| 引用本文： | 黄宁珍,付传明,赵志国,唐凤鸾,覃艳,石云平.珍稀药用植物红根草野生及离体快繁群体的遗传多样性[J].植物生理学通讯,2011(10):994-1002. |
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| 作者姓名： | 黄宁珍 付传明 赵志国 唐凤鸾 覃艳 石云平 |
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| 作者单位： | 广西壮族自治区/中国科学院广西植物研究所; |
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| 基金项目： | 广西科技攻关项目(桂科攻0322024-3B和桂科攻0992003B-31) |
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| 摘 要： | 红根草是一个有重要药用价值的珍稀濒危药材植物。为了更好地了解红根草野生和组培快繁种质的遗传多样性信息,本文用RAPD和ISSR分子标记技术,对红根草4个野生种群及一个离体快繁群体进行遗传多样性分析,为物种保护和繁育提供理论依据。结果显示,在物种水平上,该物种的遗传多样性水平中等,Nei基因多样性指数（H）、Shannon信息多样性指数（I）和多态性位点百分率（PPL）分别为0.237/0.248（RAPD/ISSR,下同）、0.365/0.380和78.4%/81.1%;遗传变异大多数（70.5%/81.5%）发生在种群内、少部分（29.5%/18.5%）发生在种群间;野生种群的基因流为1.21/2.20,但UPGMA聚类分析结果表明,距离35km以上的种群遗传分化明显,因此推测基因流动主要存在于种群内,地理距离是种群分化的主要原因。在居群水平上,H、I和PPL三项遗传多样性参数分别为0.167/0.202、0.253/0.303和51.7%/58.0%;离体快繁群体的RAPD分析结果显示,其遗传多样性高于其原野生种群,这一结果暗示,离体快繁过程中可能发生了体细胞变异,这些变异与RAPD-PCR区域有关。
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| 关 键 词： | 红根草 遗传多样性 离体快繁 体细胞变异 RAPD ISSR |
Genetic Diversity of Wild and in vitro Proliferation Plants of Salvia prionitis Hance,a Rare Chinese Herb |
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| HUANG Ning-Zhen,FU Chuan-Ming,ZHAO Zhi-Guo,TANG Feng-Luan,QIN Yan,SHI Yun-Ping Guangxi Institute of Botany,Guangxi Zhuangzu Autonomous Region , Chinese Academy of Sciences,Guilin,Guangxi ,China.Genetic Diversity of Wild and in vitro Proliferation Plants of Salvia prionitis Hance,a Rare Chinese Herb[J].Plant Physiology Communications,2011(10):994-1002. |
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| Authors: | HUANG Ning-Zhen FU Chuan-Ming ZHAO Zhi-Guo TANG Feng-Luan QIN Yan SHI Yun-Ping Guangxi Institute of Botany Guangxi Zhuangzu Autonomous Region Chinese Academy of Sciences Guilin Guangxi China |
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| Institution: | HUANG Ning-Zhen*,FU Chuan-Ming,ZHAO Zhi-Guo,TANG Feng-Luan,QIN Yan,SHI Yun-Ping Guangxi Institute of Botany,Guangxi Zhuangzu Autonomous Region and Chinese Academy of Sciences,Guilin,Guangxi 541006,China |
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| Abstract: | Salvia prionitis Hance is an endangered and valuable Chinese herb in south of China.In this study,the genetic diversity of four wild and one in vitro proliferation populations of S.prionitis was tested by using RAPD(random amplified polymorphic DNA) and ISSR(inter simple sequence repeat) markers for finding out genetic diversity information of wild and tissue culture source material and providing theory foundation for species protection and proliferation.RAPDs/ISSRs results indicated that the genetic divers... |
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| Keywords: | Salvia prionitis Hance genetic diversity in vitro proliferation somatic mutations RAPD ISSR |
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