| 过量表达星星草PtSOS_1提高拟南芥的耐盐性 |
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| 引用本文： | 程玉祥.过量表达星星草PtSOS_1提高拟南芥的耐盐性[J].植物生理学通讯,2008,44(6). |
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| 作者姓名： | 程玉祥 |
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| 作者单位： | 东北林业大学园林学院,哈尔滨,150040 |
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| 基金项目： | 黑龙江省自然科学基金
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哈尔滨市青年科学研究基金
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| 摘 要： | 将星星草中分离的质膜型Na+/H+逆向转运蛋白基因PtSOS1(GenBank登录号EF440291)构建到pGWB2植物表达载体上,转化拟南芥,获得抗卡那霉素的抗性植株.PCR和Northern检测表明,PtSOS1已整合到拟南芥基因组中并过量表达.耐盐性实验表明,PtSOS1过量表达提高了拟南芥植株的耐盐性.盐分测定表明,盐胁迫下PtSOS1转基因植株中Na+积累低于野生型的,K+含量则高于野生型的,转基因植株中K+/Na+比值高于野生型.
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| 关 键 词： | 星星草 转基因拟南芥 盐胁迫 |
Overexpression of the PtSOS_1 from Puccinellia tenuiflora Enhanced Salt Tolerance of Arabidopsis |
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| CHENG Yu-Xiang.Overexpression of the PtSOS_1 from Puccinellia tenuiflora Enhanced Salt Tolerance of Arabidopsis[J].Plant Physiology Communications,2008,44(6). |
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| Authors: | CHENG Yu-Xiang |
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| Institution: | CHENG Yu-Xiang~* College of L,scape Architecture,Northeast Forestry University,Harbin 150040,China |
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| Abstract: | The cDNAs of PtSOS_1(GenBank accession No.EF440291),a plasma membrane Na~+/H~+ antiporter gene isolated from Puccinellia tenuiflora,were constructed into plant expression vector pGWB_2.Arabidopsis thaliana wild-type(WT) plants were transformed with Agrobacterium tumefaciens containing the resultant vectors,and kanamycin-resistant plants were obtained.PCR analysis showed that PtSOS_1 was integrated in the genome of Arabidopsis.Northern analysis indicated that transgenic plants over-expressed the PtSOS_1 in t... |
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| Keywords: | PtSOS1 |
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