| 银杏叶绿体petD基因的克隆与表达 |
| |
| 引用本文： | 许锋,张威威,唐寅,王燕,程水源.银杏叶绿体petD基因的克隆与表达[J].植物生理学通讯,2010(1):37-41. |
| |
| 作者姓名： | 许锋 张威威 唐寅 王燕 程水源 |
| |
| 作者单位： | 长江大学园艺园林学院;黄冈师范学院生命科学与工程学院; |
| |
| 基金项目： | 湖北省自然科学基金(2008CDA061); 湖北省教育厅科学研究计划优秀中青年人才项目(Q20091201); 长江大学博士基金(0010113) |
| |
| 摘 要： | 根据黑松、云杉、菠菜与玉米叶绿体petD基因序列设计引物，以银杏叶绿体基因组DNA为模板，PCR扩增克隆了银杏叶绿体petD基因（GenBank登录号为DQ923066，命名为GbpetD）的序列。序列分析显示，GbpetD基因组DNA序列编码区长1243bp，含1个内含子和2个外显子，其外显子序列编码177个氨基酸。相似性比对显示，该基因编码区序列与云杉、台东苏铁、黑松、莴苣、木薯、北美落叶松的petD基因核苷酸同源性为84％-99％，氨基酸序列同源性为85％-93％。系统进化树分析结果表明GbpetD蛋白质与黑松、北美落叶松、云杉、苏铁等裸子植物的petD蛋白质聚类关系最近。半定量RT—PCR分析表明，GbpetD基因在银杏叶和茎中表达，在叶中表达量最大。
|
| 关 键 词： | 银杏 GbpetD基因 序列分析 表达 |
Cloning and Expression of A Chloroplast petD Gene from Ginkgo biloba L. |
| |
| XU Feng,ZHANG Wei-Wei,TANG Yin,WANG Yan,CHENG Shui-Yuan,College of Horticulture , Garden,Yangtze University,Jingzhou,Hubei ,China,College of Life Science , Engineering,Huanggang Normal University,Huanggang,Hubei.Cloning and Expression of A Chloroplast petD Gene from Ginkgo biloba L.[J].Plant Physiology Communications,2010(1):37-41. |
| |
| Authors: | XU Feng ZHANG Wei-Wei TANG Yin WANG Yan CHENG Shui-Yuan College of Horticulture Garden Yangtze University Jingzhou Hubei China College of Life Science Engineering Huanggang Normal University Huanggang Hubei |
| |
| Institution: | XU Feng1,ZHANG Wei-Wei1,TANG Yin1,WANG Yan1,CHENG Shui-Yuan2,1College of Horticulture , Garden,Yangtze University,Jingzhou,Hubei 434025,China,2College of Life Science , Engineering,Huanggang Normal University,Huanggang,Hubei 438000 |
| |
| Abstract: | A chloroplast petD gene,named GbpetD,was cloned from Ginkgo biloba using a pair of primers,which were designed according to the homologous sequences in Pinus thunbergii,Picea abies,Spinacia oleracea,and Zea mays.The genomic DNA of GbpetD had 1 243 bp coding sequence,including one intron and two exons,which encoding a 177-amino-acid protein.The Blast results showed that the homology of the GbpetD nucleotide sequence with petD genes from P.abies,Cycas taitungensis,P.thunbergii,Lactuca sativa,Manihot esculenta... |
| |
| Keywords: | Ginkgo biloba GbpetD sequence analysis expression |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
