续随子的组织培养与快速繁殖

本文对能源植物续随子（Euphorbia lathyris L．）进行离体培养与快速繁殖技术研究。结果表明：以种子、茎段和顶芽为外植体均能诱导获得无菌苗，其中以茎段或顶芽的芽诱导率较高；幼芽继代增殖的最佳培养基是White＋TDZ0．05mg·L。＋IBA0．01mg·L^（-1）＋0．1％活性炭，培养25d的增殖系数约为4．5；而最适生根培养基为1／2MS＋NAA1．0mg．L^（-1）＋0．1％活性炭，炼苗移栽后，成活率约为60％。培养过程中，通过控制细胞分裂素浓度能有效解决续随子培养中的玻璃化问题，添加0．1％的活性炭对缓解外植体和无菌苗褐化效果较好。

Tissue Culture and Rapid Propagation of Euphorbia lathyris L.

The technique of the tissue culture and rapid propagation of Euphorbia lathyris were studied. The results showed that the seeds, stems and terminal buds as explants could be induced to sterilized seedings, in which the bud induction rates of stems and the terminal buds were higher. The best medium for multiplication was White＋0.05 mg·L^（-1） TDZ＋0.01 mg·L^（-1） IBA＋0.1% AC, and the multiplication coefficient could be 4.5 after 25 d. The best medium for rooting was 1/2MS＋1.0 mg.L^（-1）NAA＋0.1% AC. The survival rates reached 60% after transplanting. Vitrification problem in tissue culture of Euphorbia lathyris could be effectively solved by the control of the cytokinin concentration, and materials browning could be alleviated by adding 0.1% AC.