不同光质对烟草叶片组织结构及Rubisco羧化酶活性和rbc、rca基因表达的影响

通过对烟草植株覆盖白、红、黄、蓝、紫色滤膜获得不同光质,研究了烟草叶片在7～70d的生长发育期内,不同光质处理对烟叶组织结构特征、核酮糖1,5-二磷酸羧化酶/加氧酶（Rubisco）羧化酶活性、Rubisco基因（rbc）表达及其活化酶（Rca）基因（rca）表达的影响。结果表明,与黄膜处理下生长的烟叶相比,红、蓝、紫膜处理下生长的烟叶有较高的叶片厚度、栅栏组织厚度、海绵组织厚度、栅栏细胞密度和较小的组织空隙率。此外,红、蓝、紫膜处理的叶片有较高的Rubis-co羧化酶活性和净光合速率及较强的rbc和rca基因表达。实验结果表明不同光质对烟草叶片的组织结构特征有显著影响,光质可能通过影响Rubisco羧化酶活性进而影响叶片光合效率,而光质、叶片组织结构和光合效率之间存在某种程度的相互联系。

Effects of Different Light Quality on Anatomical Structure,Carboxylase Activity of Ribulose 1,5-Biphosphate Carboxylase/Oxygenase and Expression of rbc and rca Genes in Tobacco（Nicotiana tabacum L.） Leaves

By covering tobacco plants with white,red,yellow,blue and purple films to obtain different light quality,the effects of light quality on anatomical structure,carboxylase activity of ribulose 1,5-biphosphate carboxylase/oxygenase（Rubisco）,and expression of rbc and rca genes were investigated in tobacco leaves aged from 7 to 70 days.Compared to the leaves grown under yellow film,red,blue and purple film treatments increased the leaf thickness,palisade and spongy parenchyma thickness,and density of palisade cells,but lowered the tissue interspace in the tobacco leaves.In addition,the leaves with red,blue and purple film treatments showed higher carboxylase activity of Rubisco and net photosynthetic rate and stronger expression of rbc and rca genes than those with yellow film treatment.These results indicated that,different light quality obviously influenced the anatomical structure,carboxylase activity of Rubisco and further photosynthesis efficiency of tobacco leaves,and there was a possible relationship among light quality,anatomical structure and photosynthesis efficiency of tobacco leaves to some extent.