新型甘蓝型油菜温敏核不育系SP2S的花药发育解剖学观察

SP2S是西北农林科技大学选育的甘蓝型油菜温敏核不育系,本文采用半薄树脂切片、扫描电镜对SP2S及其可育近等基因系SP2F的花药发育及花粉形态进行观察比较,发现SP2S花药发育在减数分裂时期出现异常,单核花粉时期彻底败育。其主要特征是：减数分裂时期绒毡层已经径向肥大且出现大液泡,胼胝质不能及时降解,使得单核小孢子相互粘连在一起,小孢子无花粉壁的形成且细胞质物质逐渐降解,最后小孢子仅剩下空壳残留物,聚集在一起。SP2S败育特征与现有的核不育材料不同,表明其有可能是一种新型温敏核不育材料。

Anatomical Observation of Anther Development of a New Thermo-Sensitive Genic Male Sterile Line SP2S in Brassica napus L.

SP2S is a new type thermo-sensitive genic male sterile line of Brassica napus breeding in Northwest AF University.The anatomical characters of pollen and the anther development of SP2S at different stages were compared with its near isogenic line SP2F by semi-thin sections and scan electronic microscopy.The result indicated that the anther abortion occurred at the stage of meiosis and the anther completely aborted at the later stage of uni-nucleate.The tapetal cells were highly vacuolated and deformed at the stage of meiosis.Callose around the tetrads couldn＇t be degradated timely,which made the microspores adhesion with each other.And then the microspores didn＇t form the extine normally.The cytoplasm of pollen cells decayed gradually and at last became empty.These evidences obviously indicate that SP2S is probably a new thermosensitive male sterile line which is different from the previously materials.