大花卷丹的组织培养及限制生长保存

离体保存是植物种质保存的重要手段之一,为实现对大花卷丹的保护性利用,本文对其组织培养体系及限制生长离体保存技术进行了研究。结果表明,在常温（23±2）℃、光照强度约为40μmol.m-2.s-1、光照时间14h.d-1的条件下,大花卷丹鳞片在MS＋6-BA1.0mg.L-1＋NAA0.2mg.L-1培养基中生长情况较好,能直接诱导芽,且小鳞茎的生长速度较快。将诱导出的小鳞茎切割后,接种到1/2MS＋NAA0.5mg.L-1＋活性炭2g.L-1生根培养基2～3周即能生根,生长状况良好。提高MS培养基中蔗糖达90和110g.L-1时可以抑制其生长,能够保存大花卷丹试管苗10个月,保存过程中生长正常,株高生长缓慢,但根长势较快。在蔗糖浓度90g.L-1基础上再添加30g.L-1甘露醇的培养基能进一步抑制试管苗根的生长。6个月后,转移到正常培养基上培养均能恢复生长,其鳞片在诱导培养基上能正常分化。因此,采用大花卷丹鳞片组织培养可以形成种苗,在培养基中添加高蔗糖浓度和甘露醇可以使其试管苗保存1年以上。

In vitro Propagation and Restrict Growth Preservation of Lilium leichtlinii var.maximowiczii Baker

In vitro propagation and preservation,one of the major methods for plant germplasm preservation,of Lilium leichtlinii var.maximowiczii is reported.The report shows that MS basic medium supplemented with 6-BA 1.0 g.L-1 and NAA 0.2 g.L-1 is the optimum culture for the scale.The small bulbs grow on the 1/2MS（the concentration of macroelements halved） added NAA 0.5 mg.L-1 and 2 g.L-1AC for rooting.The plantlet grew slowly on the MS medium with high concentration of sucrose to 90 and 110 g.L-1 for 10-month,and grew even more slowly added 90 g.L-1 sucrose and another 30 g.L-1 mannitol.After 6 months,the plantlet can recover growth on normal medium and the scales can induce shoots on induction medium.L.leichtlinii var.maximowiczii plantlet introducing from scales tissue culture can be preservated in MS medium with high concentration of sucrose and mannitol at least one year.