| 水稻NADP-苹果酸酶的原核表达、纯化和抗体制备 |
| |
| 引用本文： | 程玉祥,柳参奎.水稻NADP-苹果酸酶的原核表达、纯化和抗体制备[J].植物生理学通讯,2007,43(5):847-851. |
| |
| 作者姓名： | 程玉祥 柳参奎 |
| |
| 作者单位： | 东北林业大学盐碱地生物资源环境研究中心 哈尔滨150040 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划);东北林业大学校科研和教改项目 |
| |
| 摘 要： | 构建了水稻NADP-ME_2基因cDNA的原核表达载体pQE30,并诱导表达出有生物学功能的融合蛋白。用Ni-NTA琼脂糖亲和层析纯化出NADP-ME_2融合蛋白,并测定了融合蛋白酶学特性(V_(max)、K_m、K_(cat)、底物特异性)。用纯化的NADP- ME_2融合蛋白免疫家兔,制备出抗水稻NADP-ME特异性抗体。全蛋白双向电泳后Western印迹表明水稻中至少有4个NADP-ME家族蛋白质成员。
|
| 关 键 词： | 水稻 原核表达 酶学特性 抗体 |
| 修稿时间： | 2007-06-26 |
Prokaryotic Expression,Purification and Antibody Production of Rice(Oryza sativa L.)NADP-Malic Enzyme |
| |
| CHENG Yu-Xiang,LIU Shen-Kui.Prokaryotic Expression,Purification and Antibody Production of Rice(Oryza sativa L.)NADP-Malic Enzyme[J].Plant Physiology Communications,2007,43(5):847-851. |
| |
| Authors: | CHENG Yu-Xiang LIU Shen-Kui |
| |
| Abstract: | Rice NADP-ME_2 cDNA was constructed into prokaryotic expression vector(pQE30),and its bio- logically active fusion protein was expressed.NADP-ME_2 fusion protein was purified by Ni-NTA agarose affinity chromatography,and its enzyme properties such as V_(max),K_m,K_(cat),and substrate specificity were detected.Purified NADP-ME_2 fusion protein was used to immunolize rabbit.Anti-rice NADP-ME specific antibody was obtained.Four members of NADP-ME family protein had been detected by Western blot from rice total proteins after two-dimensional gel electrophoresis. |
| |
| Keywords: | NADP-ME2 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
