| ‘津田’芜菁Transparent Testa Glabra 1（BrTTG1）基因的克隆及表达分析 |
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| 引用本文： | 闫海芳,李玉花,刘振华,闵远琴.‘津田’芜菁Transparent Testa Glabra 1（BrTTG1）基因的克隆及表达分析[J].植物生理学通讯,2011(4):373-378. |
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| 作者姓名： | 闫海芳 李玉花 刘振华 闵远琴 |
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| 作者单位： | 东北林业大学生命科学学院; |
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| 基金项目： | 国家自然科学基金重点项目(30730078); 中央高校基本科研业务费专项资金(DL09BA09) |
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| 摘 要： | TTG1（Transparent Testa Glabra 1）蛋白是一种WD40类蛋白,参与植物的生长和发育。采用RT-PCR方法从芜菁品种‘津田＇中克隆了BrTTG1 cDNA序列（GenBank登录号HM208590）。该基因cDNA开放阅读框长度为1 014 bp,编码一个由337个氨基酸残基组成的蛋白,该蛋白分子量为37.28 kDa,理论等电点为4.66。与其他植物中的TTG1蛋白进行同源性比对结果显示,BrTTG1与甘蓝型油菜的TTG1同源性最高。BrTTG1蛋白在31～337位氨基酸处含有WD40超家族的保守结构域。荧光定量PCR检测BrTTG1在‘津田＇芜菁不同组织中的表达结果表明,该基因在有花青素合成的红色‘津田＇芜菁根皮中表达量最高。
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| 关 键 词： | ‘津田’芜菁 TTG1 基因克隆 表达分析 |
Cloning and Expression Analysis of Transparent Testa Glabra 1(BrTTG1) in Brassica rapa L.cv.'Tsuda' |
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| YAN Hai-Fang,LI Yu-Hua,LIU Zhen-Hua,MIN Yuan-Qin College of Life Sciences,Northeast Forestry University,Harbin ,China.Cloning and Expression Analysis of Transparent Testa Glabra 1(BrTTG1) in Brassica rapa L.cv.'Tsuda'[J].Plant Physiology Communications,2011(4):373-378. |
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| Authors: | YAN Hai-Fang LI Yu-Hua LIU Zhen-Hua MIN Yuan-Qin College of Life Sciences Northeast Forestry University Harbin China |
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| Institution: | YAN Hai-Fang,LI Yu-Hua~*,LIU Zhen-Hua,MIN Yuan-Qin College of Life Sciences,Northeast Forestry University,Harbin 150040,China |
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| Abstract: | TTG1(transparent testa glabra 1) is a WD40 protein that is involved in plant growth and development. Here,a homologue of transparent testa glabra 1 gene,designated BrTTG1,was isolated from 'Tsuda' (Brassica rapa L.) by RT-PCR(GenBank accession number HM208590).The ORF of the BrTTG1 was 1 014 bp long and encoded a putative protein of 337 amino acids with a molecular weight of 37.28 kDa and a theoretical pI of 4.66.BrTTG1 protein contained a WD40 superfamily conserved domain and had a high identity with TTG1 ... |
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| Keywords: | Brassica rapa L cv 'Tsuda' TTG1 gene cloning expressing analysis |
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