| 叠氮化钠诱变玉扇愈伤组织的研究 |
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| 引用本文： | 吴正景,张菊平,时灿辉,巩振辉.叠氮化钠诱变玉扇愈伤组织的研究[J].植物生理学通讯,2010(12):1247-1250. |
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| 作者姓名： | 吴正景 张菊平 时灿辉 巩振辉 |
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| 作者单位： | [1]河南科技大学林学院,河南洛阳471003 [2]西北农林科技大学园艺学院,陕西杨凌712100 |
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| 基金项目： | 陕西省“13115”科技创新工程重大科技专项（2007ZDKG-05）; 河南科技大学博士科研基金（09001216） |
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| 摘 要： | 在含不同浓度叠氮化钠（0、0.01、0.05、0.1、0.5和1.0mmol·L~（-1））的诱导培养基上诱导玉扇愈伤组织发生变异。结果表明,叠氮化钠对玉扇愈伤组织的半致死浓度为0.1～0.5 mmo1·L~（-1）,0.1 mmol·L~（-1）有利于保证一定量的有效变异率和再生率。再生芽多发生叶片失绿、畸形和叶序改变,其中较多的叶序变异,有望为研究叶序决定提供材料。
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| 关 键 词： | 玉扇 叠氮化钠 诱变 叶序 |
Study of Sodium Azide Inducing Effect on Callus of Haworthia Truncata var. truncata |
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| WU Zheng-Jing,＊,ZHANG Ju-Ping,SHI Can-Hui,GONG Zhen-Hui.Study of Sodium Azide Inducing Effect on Callus of Haworthia Truncata var. truncata[J].Plant Physiology Communications,2010(12):1247-1250. |
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| Authors: | WU Zheng-Jing ＊ ZHANG Ju-Ping SHI Can-Hui GONG Zhen-Hui |
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| Institution: | 1 College of Forestry,Henan University of Science and Technology,Luoyang,Henan 471003,China;2 College of Horticulture, Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100,China |
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| Abstract: | In this study sodium azide（0,0.01,0.05,0.1,0.5,1.0 mmol·L~（-1） in tissue culture medium） was used to induce callus of Haworthia truncata.The results showed that the half-death dosage of sodium azide to Haworthra truncata callus was between 0.1 and 0.5 mmol·L~（-1）,but 0.1 mmol·L~（-1） sodium azide was able to insure some useful variations that mostly were leaf color,shape and phyllotaxis variation,among all the mutations, phyllotaxis variation could provide material to study leaf arrangement. |
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| Keywords: | Haworthia truncata sodium azide inducement phyllotaxis |
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