LED不同光质对萝卜愈伤组织诱导、增殖和萝卜硫素含量的影响

以白水萝卜无菌苗及其愈伤组织为实验材料,研究其在LED白、红、黄、蓝、绿和蓝红6种光质下的愈伤诱导和增殖。结果表明：LED不同光质下胚轴愈伤组织的诱导效应不同,诱导率顺序依次为黄光〉红光〉蓝红光〉白光〉蓝光〉绿光;蓝光、黄光和红光有利于子叶愈伤组织的诱导;子叶诱导愈伤组织的效果较下胚轴好;LED红光下愈伤组织的增殖倍数和萝卜硫素含量均为最高。

Effects of Different LED Light Qualities on Callus Induction,Proliferation and Sulforaphane Content of Raphanus sativus L.

The callus induction and proliferation times of white radish （Raphanus sativus） under 6 kinds of LED light qualities （red, yellow, green, blue, white and blue＋red） were investigated in this experiment. Results showed that different LED light qualities had different effects on callus induction of hypocotyls, with the efficiency in order of yellowredblue＋red whitebluegreen, and the blue light, the yellow light and the blue＋red light were all in favor of the callus induction of cotyledons, that more callus was induced from cotyledons than from hypocotyls, and that LED red light was the most effective light quality for callus proliferation and sulforaphane accumulation.