茶树冷诱导基因RAV的克隆与表达特性分析

对利用cDNA-AFLP技术所获得的茶树低温诱导差异表达片段TDF,通过RACE方法获得含完整编码区序列的茶树RAV基因cDNA克隆,其开放阅读框编码361个氨基酸,包含两个保守的结构域AP2和B3,与多种植物RAV蛋白具有高度同源性。qRT-PCR分析表明,茶树RAV基因受低温、乙烯、NaCl等上调表达,最大表达量分别是诱导前的5.8、10.0和1.9倍。在成熟叶片、芽、嫩茎中RAV基因表达量相近,花蕾和嫩根中表达较低,而在种子中不表达。推测该基因在组织中的表达受到严格控制以及在响应非生物胁迫中发挥重要作用。

Cloning and Expression Analysis of RAV Gene Related to Cold Stress from Tea Plant [Camellia sinensis （L.） O.Kuntz]

For a TDF, which has been gained from genes expressed differentially in tea plant under cold stress using cDNA-AFLP, containing a complete coding sequence cDNA was cloned by RACE, named CsRAV, and contains an ORF, which encodes a polypeptide of 361 amino acids including two conserved domains： AP2 and B3. Sequence alignment showed that CsRAV protein shared high identity with other plants. qRT-PCR results indicated that CsRAV was induced by cold, ethylene and NaCl, and maximum relative expression was 5.8, 10.0 and 1.9 times higher than before treatment, respectively. CsRAV gene expression level was closed in leave, bud and stem, and lower in flower and root, not detected in seed. It shows the expression of the CsRAV might be strictly controlled in different organs and plays an important role during abiotic stress in tea plant.