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拟南芥RopGEFs家族基因在外源脱落酸处理下的表达分析
引用本文:王会,金宇騑,卢曼曼,葛光君,向芬,李杜,朱咏华,郑巧林.拟南芥RopGEFs家族基因在外源脱落酸处理下的表达分析[J].植物生理学通讯,2010(8):817-823.
作者姓名:王会  金宇騑  卢曼曼  葛光君  向芬  李杜  朱咏华  郑巧林
作者单位:湖南大学生物学院生物技术系,长沙410082
基金项目:湖南省科技计划项目(2009FJ3198).
摘    要:ABA不同浓度和不同时间处理拟南芥野生型7天龄幼苗,采用实时荧光定量PCR方法,分析小G蛋白ROP10和ROP乌苷酸交换因子RopGEFs基因的表达水平差异。结果表明,RopGEF7-10,12及33没有检测到表达,而RopGEFI-6,11,14ROP10在ABA处理下表现出不同的应答趋势,其中RopGEF5基因的表达变化与ROP10的变化相似。推测RopGEF5有可能在ROP10介导的ABA信号转导途经中起调控作用。

关 键 词:拟南芥  脱落酸  ROP10  RopGEFs

Expression Analysis of RopGEFs Genes in Arabidopsis thaliana Treated with ABA
WANG Hui,JIN Yu-Fei,LU Man-Man,GE Guang-Jun,XIANG Fen,LI Du,ZHU Yong-Hua,ZHENG Qiao-Lin.Expression Analysis of RopGEFs Genes in Arabidopsis thaliana Treated with ABA[J].Plant Physiology Communications,2010(8):817-823.
Authors:WANG Hui  JIN Yu-Fei  LU Man-Man  GE Guang-Jun  XIANG Fen  LI Du  ZHU Yong-Hua  ZHENG Qiao-Lin
Institution:( Department of Biotechnology, College of Biology, Hunan University, Changsha 410082, China)
Abstract:The real-time fluorescence quantitative PCR was carried out to analyze the expression profiles of ROP10 (Rho-related GTPase from plants) and RopGEFs (guanine nucleotide exchange factor) genes in 7-d seedlings of Arabidopsis thaliana in response to ABA with different concentrations or periods. The results showed that the expression of RopGEF7-10, 12 and 13 could not be detected in wild type (Col-4) seedlings. RopGEF1-6, 11, 14 and ROPIO indicated different expression patterns in response to ABA treatment, while RopGEF5 showed similar expression profile to ROP10. These results suggested that RopGEF5 might act as a regulate factor in ROP10-dependent ABA signaling pathway.
Keywords:Arabidopsis thaliana  ABA  ROP10  RopGEFs
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