| 水杨酸诱导的烟草抗性相关序列的分离 |
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| 引用本文： | 董霞,李文正,付坚,兰建强,李正风,刘世贵.水杨酸诱导的烟草抗性相关序列的分离[J].植物生理学通讯,2007,43(4):711-715. |
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| 作者姓名： | 董霞 李文正 付坚 兰建强 李正风 刘世贵 |
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| 作者单位： | 四川大学生命科学学院，云南省烟草科学研究所，云南省农业科学院生物技术与种质资源研究所，云南农业大学食品科学技术学院，云南省烟草科学研究所，四川大学生命科学学院 成都610064 云南农业大学食品科学技术学院，昆明650201，云南玉溪653100，昆明650223，昆明650201，云南玉溪653100，成都610064 |
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| 基金项目： | 国家自然科学基金;云南省烟草公司资助项目 |
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| 摘 要： | 通过已分离鉴定的水杨酸诱导烟草‘云烟85’中与抗性相关的差异表达基因,采用差示筛选和反式Northern检测以及序列分析得到94个烟草差异表达的EST序列。经测序及同源性比较,其中87个有同源序列,7个为新序列;有51个与抗性相关,占总序列的54.3%,其中有系统获得抗性蛋白基因和病程相关蛋白基因等。
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| 关 键 词： | 水杨酸 烟草 差示筛选 序列分析 抗性相关基因 |
| 修稿时间： | 2007-04-29 |
Isolation of Resistance-related Genes Induced by Salicylic Acid from Nicotiana tabacum L. |
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| DONG Xia,LI Wen-Zheng,FU Jian,LAN Jian-Qiang,LI Zheng-Feng,LIU Shi-Gui.Isolation of Resistance-related Genes Induced by Salicylic Acid from Nicotiana tabacum L.[J].Plant Physiology Communications,2007,43(4):711-715. |
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| Authors: | DONG Xia LI Wen-Zheng FU Jian LAN Jian-Qiang LI Zheng-Feng LIU Shi-Gui |
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| Institution: | 1 College of Life Sciences, Sichuan University, Chengdu 610064, China; 2 Yunnan Tobacco Science Research Institute, Yuxi, Yunnan 653100, China; 3 Faculty of Food Science, Yunnan Agricultural University, Kunming 650201, China; 4 Biotechnology and Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China |
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| Abstract: | To clarify mechanisms of salicylic acid (SA) induction in tobacco, differential expression resistance-related genes were isolated from suppression subtractive hybridization (SSH) library induced by SA in tobacco 'Yunyan 85'. 94 clones were selected by differential screening and virtual northern analysis. The blastn homology search analysis revealed that 87 clones had homologue genes, and 7 clones were new. And there were 51 clones related with resistance, accounting for 54.3% of total expression genes. Most of them involved in the process of systemic acquired resistance protein and pathogenesis-related protein. |
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| Keywords: | salicylic acid Nicotiana tabacum differential screening sequence analysis resistance-related gene |
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