| 类大麦材料y型高分子量谷蛋白基因不表达的鉴定 |
| |
| 引用本文： | 颜泽洪,郭志富,代寿芬,魏育明,郑有良.类大麦材料y型高分子量谷蛋白基因不表达的鉴定[J].植物生理学通讯,2005,41(6):745-748. |
| |
| 作者姓名： | 颜泽洪 郭志富 代寿芬 魏育明 郑有良 |
| |
| 作者单位： | 四川农业大学小麦研究所,四川,都江堰,611830 |
| |
| 基金项目： | 全国高等学校优秀博士学位论文作者专项基金，重庆市应用基础研究基金，中国科学院资助项目，四川省青年科技基金，四川省科技厅资助项目 |
| |
| 摘 要： | 利用SDS-PAGE检测了2份类大麦属植物的高分子量谷蛋白亚基组成,在小麦的高分子量区域仅检测到1条蛋白带,因此怀疑其y型亚基没有表达.根据其它高分子量谷蛋白提取方法的结果以及基因编码区部分序列测定,确认其y型高分子量谷蛋白基因是沉默的.
|
| 关 键 词： | 类大麦属 高分子量谷蛋白基因 基因沉默 |
| 收稿时间： | 2005-04-07 |
| 修稿时间： | 2005-09-26 |
Identification of An Inactive y-Type High-Molecular-Weight Glutenin Gene in Crithopsis delileana (Schult.) Roshev. |
| |
| YAN Ze-Hong,GUO Zhi-Fu,DAI Shou-Fen,WEI Yu-Ming,ZHENG You-Liang.Identification of An Inactive y-Type High-Molecular-Weight Glutenin Gene in Crithopsis delileana (Schult.) Roshev.[J].Plant Physiology Communications,2005,41(6):745-748. |
| |
| Authors: | YAN Ze-Hong GUO Zhi-Fu DAI Shou-Fen WEI Yu-Ming ZHENG You-Liang |
| |
| Institution: | Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, Sichuan 611830, China |
| |
| Abstract: | Using SDS-PAGE analysis, the high-molecular-weight glutenin subunits (HMW-GS) of two Crithopsis delileana (Schult.) Roshev. accessions were detected. It was found that the two accessions had the same HMW-GS. Only one HMW-GS with the similar electrophoresis mobility to the y-type HMW-GS of hexaploid wheat was observed in C. delileana. It was not sure whether the y-type HMW glutenin genes were silenced or not. By using other protein extraction methods and sequencing of partial gene coding region, it was confirmed that, in fact, the y-type HMW glutenin gene in C. delileana accessions was silenced. |
| |
| Keywords: | Crithopsis delileana (Schult ) Roshev high-molecular-weight (HMW) glutenin gene gene silence |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
