| 同源四倍体铁皮石斛的生长及多糖积累 |
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| 引用本文： | 江金兰,叶炜,李永清,周建金,雷伏贵,魏道智.同源四倍体铁皮石斛的生长及多糖积累[J].植物生理学通讯,2014(4):519-526. |
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| 作者姓名： | 江金兰 叶炜 李永清 周建金 雷伏贵 魏道智 |
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| 作者单位： | [1]三明市农业科学研究院药用植物研究所,福建沙县365509 [2]福建农林大学生命科学学院,福州350002 |
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| 基金项目： | 三明市科技计划项目（2011-K-1）和三明市农业科学研究院院立项目（2013-Y-01）. |
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| 摘 要： | 通过秋水仙素诱导铁皮石斛多倍体，以外观形态、气孔、染色体数目筛选多倍体植株，分析多倍化对铁皮石斛多糖累积及生长的影响。结果表明，同源四倍体铁皮石斛茎、叶及原球茎的多糖含量分别是二倍体的1.31、1．83及1．95倍，但生长速度显著或极显著低于对照。ISSR分析显示，同源四倍体植株产生较明显的基因变异。
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| 关 键 词： | 铁皮石斛 多倍体 ISSR 多糖 |
Growth and Polysaccharides Accumulation in Autotetraploid Dendrobium officinale |
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| JIANG Jin-Lan,YE Wei,LI Yong-Qing,ZHOU Jian-Jin,LEI Fu-Gui,WEI Dao-Zhi.Growth and Polysaccharides Accumulation in Autotetraploid Dendrobium officinale[J].Plant Physiology Communications,2014(4):519-526. |
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| Authors: | JIANG Jin-Lan YE Wei LI Yong-Qing ZHOU Jian-Jin LEI Fu-Gui WEI Dao-Zhi |
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| Institution: | 1The Institute of Medicinal Plant, Sanming Academy of Agricultural Science, Shaxian, Fujian, 365509, China; 2College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China) |
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| Abstract: | Polyploidy Dendrobium officinale was induced by colchicine. Autotetraploid plantlets were screened by appearance, stoma and chromosome number. The accumulation of polysaccharides and growth rate in auto- tetraploid were also analyzed. Results indicated that, the contents of polysaccharides in stem, leaf, and proto- corm-like body of autotetraploid were 1.31, 1.83 and 1.95 fold to diploid, but the growth rate were significantly or very significantly lower than the control. Obviously genomic mutations were observed by ISSR molecular marker analysis in autotetraploid plants. |
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| Keywords: | Dendrobium officinale polyploid ISSR polysaccharide |
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