血叶兰的组织培养与快速繁殖

以血叶兰的嫩茎段为外植体, 建立了血叶兰的组培快繁体系。结果表明： 芽诱导最适宜的培养基是MS＋6-BA 5.0 mg·L-1; 最适芽增殖培养基为MS＋6-BA 5.0 mg·L-1＋NAA 0.5 mg·L-1＋TDZ 0.3 mg·L-1, 芽增殖率达4.92倍; 最佳壮苗培养基为MS＋NAA 0.3 mg·L-1＋GA3 1.0 mg·L-1＋15%椰汁, 芽苗高度达到4.33 cm, 芽粗为0.61 cm; 生根最适培养基为1/2MS＋IBA 1.0 mg·L-1＋15%香蕉＋0.5 g·L-1活性炭, 生根率在93.0%以上; 炼苗后, 移栽在泥炭土＋珍珠岩＋松树皮（3：1：1, V/V/V）混合基质中, 存活率高于87.0%。

Tissue Culture and Rapid Propagation of Ludisia discolor

This paper deals with tissue culture of Ludisia discolor by tender stem explants. The results showed the suitable medium of initial was MS medium supplemented with 5.0 mg·L-1 6-BA. The best proliferation occurred on MS medium supplemented with 5.0 mg·L-1 6-BA and 0.5 mg·L-1 NAA and 0.3 mg·L-1 TDZ, the propagation coefficient of adventitious shoots reached 4.92. The best hardening medium was MS medium supplemented with 0.3 mg·L-1 NAA and 1.0 mg·L-1 GA3 and 15% coconut milk, with the average plantlet height of 4.33 cm and the average stem diameter of 0.61 cm. The best rooting for plantlets occurred on 1/2MS medium supplemented with 1.0 mg·LI IBA and 15% banana and 0.5 g·L-1 acfivated carbons, with rooting rate up to 93.0%. The plantlets with acclimatization were transferred to the the mixed matrix peat soil：vermiculite：pine bark=3：l·L （V/V/V）] and the outplanting survival rate was 87.0%.