跨膜Ca2＋和H＋离子流参与里那醇诱导的拟南芥中抗虫防御反应

里那醇可诱导植物产生防御物质从而阻止昆虫及病原菌的伤害。本文以拟南芥植株为材料，研究了里那醇对小菜蛾取食选择性的影响，测定了里那醇处理下小菜蛾生长量及存活率。采用非损伤微测技术分别检测了里那醇熏蒸及未熏蒸拟南芥对小菜蛾口腔分泌物及里那醇瞬时处理的植株叶片跨膜Ca2＋和H＋流的变化。结果表明，小菜蛾幼虫对里那醇熏蒸处理的植株选择性较差，用里那醇熏蒸后的拟南芥植株喂养小菜蛾幼虫，幼虫的生长量及存活率均低于对照组。小菜蛾口腔分泌物和里那醇熏蒸处理均导致拟南芥叶组织跨膜ca2＋和H＋离子迅速外流，且小菜蛾口腔分泌物的作用更大。而对照中ca2＋和H＋离子流整体均无明显变化。研究结果证明里那醇作为防御信号参与了拟南芥对昆虫的防御反应，叶组织跨膜ca2＋和H＋离子流变化可能是拟南芥识别昆虫口腔分泌物与里那醇两种不同刺激的初始信号。

Transmembrane Calcium and Hydrogen Ion Flux Involved in Insect-Resistant Defense Induced by Linalool in Arabidopsis thaliana L.

It is documented that linalool would induce plants to produce defense substances to prevent the harm from insects and pathogens. To analyze the early events and effects on the performances on herbivorous insects caused by linalool, Arabidopsis thaliana and Plutella xylostella were used as materials and net ion fluxes of H＋ and Ca2＋, as well as selectivity, growth rate, survival rate of P. xylostella feeding on Arabidopsis seedlings with or without linalool fumigating were analyzed. The results indicated that effluxes of Ca2＋ and H＋ were rapidly induced by linalool exposure and insect oral secretions whereas insect oral secretions resulted in stronger effects. Performances ofP. xylostella were affected by linalool fumigating and lower selection, growth and survival rate were observed on linalool fumigated seedlings. Therefore, linalool might participate in insect resistance as defense substances, Ca2＋ and H＋ flux change might be primal signals in the insect-resistant defense induced by linalool in Arabidopsis.