Application of atomic force microscopy in cancer research |
| |
| Authors: | Xiangying Deng Fang Xiong Xiayu Li Bo Xiang Zheng Li Xu Wu Can Guo Xiaoling Li Yong Li Guiyuan Li Wei Xiong Zhaoyang Zeng |
| |
| Institution: | 1.The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine,Central South University,Changsha,China;2.The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Xiangya School of Medicine,Central South University,Changsha,China;3.Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital,Central South University,Changsha,China;4.Department of Chemistry,University of North Dakota,Grand Forks,USA;5.Department of Cancer Biology, Lerner Research Institute,Cleveland Clinic,Cleveland,USA |
| |
| Abstract: | Atomic force microscopy (AFM) allows for nanometer-scale investigation of cells and molecules. Recent advances have enabled its application in cancer research and diagnosis. The physicochemical properties of live cells undergo changes when their physiological conditions are altered. These physicochemical properties can therefore reflect complex physiological processes occurring in cells. When cells are in the process of carcinogenesis and stimulated by external stimuli, their morphology, elasticity, and adhesion properties may change. AFM can perform surface imaging and ultrastructural observation of live cells with atomic resolution under near-physiological conditions, collecting force spectroscopy information which allows for the study of the mechanical properties of cells. For this reason, AFM has potential to be used as a tool for high resolution research into the ultrastructure and mechanical properties of tumor cells. This review describes the working principle, working mode, and technical points of atomic force microscopy, and reviews the applications and prospects of atomic force microscopy in cancer research. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
