QDs versus Alexa: reality of promising tools for immunocytochemistry |
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| Authors: | Helena Montón Carme Nogués Emma Rossinyol Onofre Castell Mònica Roldán |
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| Institution: | 1. Servei de Microscòpia, Universitat Autònoma de Barcelona, Bellaterra Campus, 08193, Bellaterra, Barcelona, Spain
2. Departament de Biologia Cellular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, Bellaterra Campus, 08193, Bellaterra, Barcelona, Spain
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| Abstract: | Background The unique photonic properties of the recently developed fluorescent semiconductor nanocrystals (QDs) have made them a potential
tool in biological research. However, QDs are not yet a part of routine laboratory techniques. Double and triple immunocytochemistries
were performed in HeLa cell cultures with commercial CdSe QDs conjugated to antibodies. The optical characteristics, due to
which QDs can be used as immunolabels, were evaluated in terms of emission spectra, photostability and specificity. |
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