Homologous recombination is reduced in female embryonic stem cells by two active X chromosomes |
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| Authors: | Yuka Tamura Tatsuya Ohhata Hiroyuki Niida Satoshi Sakai Chiharu Uchida Kazuma Masumoto Fuminori Katou Anton Wutz Masatoshi Kitagawa |
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| Institution: | 1. Department of Molecular Biology, Hamamatsu University School of Medicine, Hamamatsu Japan ; 2. Department of Oral and Maxillofacial Surgery, Hamamatsu University School of Medicine, Hamamatsu Japan ; 3. Advanced Research Facilities & Services, Preeminent Medical Photonics Education & Research Center, Hamamatsu University School of Medicine, Hamamatsu Japan ; 4. Institute of Molecular Health Sciences, ETH Zürich, Zurich Switzerland |
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| Abstract: | The reactivation of X‐linked genes is observed in some primary breast tumors. Two active X chromosomes are also observed in female embryonic stem cells (ESCs), but whether double doses of X‐linked genes affect DNA repair efficiency remains unclear. Here, we establish isogenic female/male ESCs and show that the female ESCs are more sensitive to camptothecin and have lower gene targeting efficiency than male ESCs, suggesting that homologous recombination (HR) efficiency is reduced in female ESCs. We also generate Xist‐inducible female ESCs and show that the lower HR efficiency is restored when X chromosome inactivation is induced. Finally, we assess the X‐linked genes with a role in DNA repair and find that Brcc3 is one of the genes involved in a network promoting proper HR. Our findings link the double doses of X‐linked genes with lower DNA repair activity, and this may have relevance for common diseases in female patients, such as breast cancer. |
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| Keywords: | embryonic stem cells homologous recombination sex differences X chromosome inactivation Xist |
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