Does Manganese Protect Cultured Human Skin Fibroblasts Against Oxidative Injury by Uva, Dithranol and Hydrogen Peroxide? |
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Authors: | Marie-Odile Parat Marie-Jeanne Richard Marie Th r se Leccia Pierre Amblard Alain Favier Jean Claude B ani |
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Institution: |
a Laboratoire de Biochimie C, Laboratoire de recherchephotobiologique en dermatologie,
b Groupe de Recherche sur les Pathologies Oxydatives CHRU Albert Michallon, Grenoble, France |
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Abstract: | Reactive oxygen species (ROS) are involved in the mechanism of photoaging and carcinogenesis. Skin is endowed with antioxidant enzymes including superoxide dismutases (SOD): cytosolic copper zinc SOD and mitochondrial manganese SOD. The aim of our study was to estimate the protective effect of manganese against oxidative injury on cultured human skin fibroblasts. Dithranol, hydrogen peroxide and UV-A radiation (375 nm) were employed as oxidative stressors. The supply of manganese chloride produced an increase in cellular content of this element up to 24 fold without concomitant elevation of MnSOD activity. Nevertheless, manganese protects cells against two of the three ROS generating systems assessed, namely hydrogen peroxyde and UV-A. This protective effect depends on the concentration of manganese in the medium, 0.1 mM and 0.2 mM protect against UVA cytotoxicity, only 0.2 mM protects against H2O2 cytotoxicity. |
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Keywords: | antioxidant Manganese superoxide dismutase free radicals cytotoxicity |
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