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Increased levels of superoxide in brains from old female rats |
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| Authors: | Antier Daniel Carswell Hilary V O Brosnan M Julia Hamilton Carlene A Macrae I Mhairi Groves S Jardine E Reid John L Dominiczak Anna E |
| Institution: | Division of Cardiovascular & Medical Sciences, University of Glasgow, Glasgow, Scotland, UK. |
| Abstract: | Hypertension, aging and a range of neurodegenerative diseases are associated with increased oxidative damage. The present study examined whether superoxide (O2?-) levels in brain are increased during aging in female rats, and the role of superoxide dismutase (SOD) and oestrogen in regulating O2?- levels.
Young adult (3 month) and old (11 month) female spontaneously hypertensive stroke prone rats (SHRSP) and normotensive Wistar-Kyoto rats (WKY) were studied. O2?- levels were measured in brain homogenates by lucigenin chemiluminescence and SOD expression by Western blotting. Ageing significantly increased brain O2?- levels in WKY (cortex +216%, hippocampus +320%, striatum +225%) and to a greater extent in SHRSP (cortex +540%, hippocampus +580%, striatum +533%). Older SHRSP showed a decline in cortical Cu/Zn SOD expression compared to young adult SHRSP. Oestrogen did not attenuate O2?- levels. The results show a significant age-dependent increase in brain O2?- levels which is exaggerated in SHRSP. The excess cortical O2?- levels in the SHRSP may be associated with a down-regulation of Cu/Zn SOD but are not related to a decrease in oestrogen. |
| Keywords: | Rat Age Hypertension Superoxide Oestrogen Female |
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