Extension of Lander-Waterman theory for sequencing filtered DNA libraries |
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Authors: | Email author" target="_blank">Michael?C?WendlEmail author W?Brad?Barbazuk |
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Institution: | (1) Genome Sequencing Center, Washington University, St. Louis, MO 63108, USA;(2) Donald Danforth Plant Science Center, St. Louis, MO 63132, USA |
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Abstract: | Background The degree to which conventional DNA sequencing techniques will be successful for highly repetitive genomes is unclear. Investigators
are therefore considering various filtering methods to select against high-copy sequence in DNA clone libraries. The standard
model for random sequencing, Lander-Waterman theory, does not account for two important issues in such libraries, discontinuities
and position-based sampling biases (the so-called "edge effect"). We report an extension of the theory for analyzing such
configurations. |
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Keywords: | |
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