SolexaQA: At-a-glance quality assessment of Illumina second-generation sequencing data |
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| Authors: | Murray P Cox Daniel A Peterson Patrick J Biggs |
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| Institution: | (1) Institute of Molecular BioSciences, Massey University, Palmerston North, 4442, New Zealand;(2) The Allan Wilson Centre for Molecular Ecology and Evolution, New Zealand;(3) The Bio-Protection Research Centre, New Zealand;(4) Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, 4442, New Zealand;(5) Massey Genome Service, Massey University, Palmerston North, 4442, New Zealand |
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| Abstract: | Background Illumina's second-generation sequencing platform is playing an increasingly prominent role in modern DNA and RNA sequencing
efforts. However, rapid, simple, standardized and independent measures of run quality are currently lacking, as are tools
to process sequences for use in downstream applications based on read-level quality data. |
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