PathogenMIPer: a tool for the design of molecular inversion probes to detect multiple pathogens |
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Authors: | Sreedevi Thiyagarajan Miloslav Karhanek Michael Akhras Ronald W Davis and Nader Pourmand |
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Institution: | (1) Stanford University, Palo Alto, CA, USA |
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Abstract: | Background Here we describe PathogenMIPer, a software program for designing molecular inversion probe (MIP) oligonucleotides for use
in pathogen identification and detection. The software designs unique and specific oligonucleotide probes targeting microbial
or other genomes. The tool tailors all probe sequence components (including target-specific sequences, barcode sequences,
universal primers and restriction sites) and combines these components into ready-to-order probes for use in a MIP assay.
The system can harness the genetic variability available in an entire genome in designing specific probes for the detection
of multiple co-infections in a single tube using a MIP assay. |
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Keywords: | |
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