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| 响应面设计法优化单葡萄糖醛酸基甘草次酸（GAMG）发酵转化培养基 | |
| 引用本文： | 李丽敏,邹树平,戴大章,李春.响应面设计法优化单葡萄糖醛酸基甘草次酸（GAMG）发酵转化培养基[J].生物加工过程,2009,7(5):29-33. |
| 作者姓名： | 李丽敏 邹树平 戴大章 李春 |
| 作者单位： | 1. 北京理工大学,生命科学与技术学院,北京,100081 2. 天津大学,化工学院,天津,300072 3. 北京理工大学,生命科学与技术学院,北京,100081;天津大学,化工学院,天津,300072 |
| 基金项目： | 国家自然科学基金资助项目，北京市自然科学基金资助项目，北京理工大学基础研究基金资助项目，北京理工大学研究生科技创新项目 |
| 摘 要： | 以甘草酸（dycyfrhizin，GL）为底物，利用产紫青霉（Penicillium purpurogenum Li-3）液态发酵转化单葡萄糖醛酸甘草次酸（GAMG），采用响应面设计法对初始发酵培养基进行优化。用部分因子分析法研究原始发酵培养基各成分对响应值的显著程度，发现甘草酸（GL）、NaNO3和K2HPO4的质量浓度对发酵产生GAMG的影响显著（P〈0．01）。用中心组合设计确立甘草酸、NaNO3和K2HPO4的适宜质量浓度分别为2．8、3．0和0．8g／L。在优化条件下进行发酵时，GAMG的转化率从75．49％提高到89．11％，比优化前提高了13．62％。 |
| 关 键 词： | 单葡萄糖醛酸基甘草次酸 产紫青霉 响应面设计 |
Optimization of medium composition for glycyrrhetinic acid monoglucuronide(GAMG) production using response surface methodology |
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| LI Li-min,ZOU Shu-ping,DAI Da-zhang,LI Chun.Optimization of medium composition for glycyrrhetinic acid monoglucuronide(GAMG) production using response surface methodology[J].Chinese Journal of Bioprocess Engineering,2009,7(5):29-33. | |
| Authors: | LI Li-min ZOU Shu-ping DAI Da-zhang LI Chun |
| Institution: | 1. School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China; 2. School of Chemical Engineering,Tianjin University, Tianjin 300072, China) |
| Abstract: | Response surface methodology was used to optimize the initial medium composition for glycyrrhetinic acid monoglucuronide （GAMG） production by Penicillium purpurogenum Li-3 with glycyrrhizin as a substrate. The impact of initial medium constituents on GAMG production was analyzed by fractional factorial design. It showed that glycyrrhizin, sodium nitrate and dibasic potassium phosphate substantially influenced GAMG production （ P 〈 0.01 ）. The optimum concentrations of glycyrrhizin, sodium nitrate and dibasic potassium phosphate were confirmed by central composite design. When the medium composition （ in g/L） was glycyrrhizin 2. 8, sodium nitrate 3.0, and dibasic potassium phosphate 0. 8. The GAMG conversion rate was improved from 75.49% to 89. 11%, under the optimization conditions. |
| Keywords: | glycyrrhetinic acid monoglucuronide Penicillium purpurogenum response surface methodology |
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