桑椹中黄酮类物质的大孔树脂纯化工艺

以桑椹中黄酮类物质的吸附量和解吸率为指标,对比分析HZ-801、HZ-816、HZ-818等12种大孔吸附树脂对桑椹提取液的分离纯化效果,优选出最佳树脂HZ-801并通过对上样液pH、上样液质量浓度、上样量、吸附流速、洗脱剂质量浓度、洗脱剂用量、洗脱流速等影响因素的考察,确定最优工艺:吸附阶段上样液pH=4,上样液质量浓度0.45mg/mL,上样量420mL,吸附流速120mL/h,动态吸附量(干树脂)25.34mg/g,吸附率84.25%;洗脱阶段的洗脱剂体积分数为60%乙醇,洗脱剂用量270mL,洗脱流速120mL/h。此优化工艺条件下的洗脱率为85.78%,总黄酮纯度从23.64%提高到82.36%。

Purification of mulberry total flavonoids by macroporous resins

Macroporous resins were used for purification of the flavonoids from mulberry.The static adsorption and desorption experiments were adopted to select the proper adsorption carrier（HZ-801）.Dynamic absorption and desorption experiment were used to investigate the optimum operating conditions.The optimum parameters were as follows：the adsorption capacity 25.34 mg/g（dry resin）and the adsorption rate 84.25%,with which the optimum adsorption conditions were found to be the initial concentration of the flavonoids solution of 0.45 mg/mL,flow rate of 120 mL/h,pH of 4,and the sample volume of 420 mL.The elution rate reached 85.78% when the optimal desorption conditions were considered as a flow rate of 120 mL/h,ethanol of 60%,and ratio of eluent volume to the bed volume of 270 mL.After the column purification,the purity of flavonoids could be enhanced from 23.64% to 82.36%.