| γ-聚谷氨酸生产菌的选育及培养条件研究 |
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| 引用本文： | 冯志彬,程仕伟,缪静,张玉香,王芹芹,杨在东,朱应普.γ-聚谷氨酸生产菌的选育及培养条件研究[J].生物加工过程,2010,8(1):40-44. |
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| 作者姓名： | 冯志彬 程仕伟 缪静 张玉香 王芹芹 杨在东 朱应普 |
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| 作者单位： | 鲁东大学,生命科学学院,烟台,264025 |
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| 基金项目： | 山东省教育厅科技计划资助项目，鲁东大学学科建设经费资助项目 |
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| 摘 要： | 从土壤中筛选分离到1株γ聚谷氨酸的生产菌株yt102,初步鉴定为枯草芽孢杆菌;以此为出发菌株采用紫外线(UV)、亚硝基胍(NTG)进行复合诱变,获得1株γ聚谷氨酸高产突变株,突变株连续传代10次,发酵性能稳定;通过单因素和正交试验确定培养基的最佳组成,在最优条件下,γ聚谷氨酸的平均产量可达28.5 g/L。
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| 关 键 词： | 枯草芽孢杆菌 优化培养 γ-聚谷氨酸 筛选 |
Screening and optimizing culture conditions of Bacillus strain for producing γ-polyglutamic acid |
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| FENG Zhi-bin,CHENG Shi-wei,MIAO Jing,ZHANG Yu-xiang,WANG Qin-qin,YANG Zai-dong,ZHU Ying-pu.Screening and optimizing culture conditions of Bacillus strain for producing γ-polyglutamic acid[J].Chinese Journal of Bioprocess Engineering,2010,8(1):40-44. |
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| Authors: | FENG Zhi-bin CHENG Shi-wei MIAO Jing ZHANG Yu-xiang WANG Qin-qin YANG Zai-dong ZHU Ying-pu |
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| Institution: | FENG Zhi-bin,CHENG Shi-wei,MIAO Jing,ZHANG Yu-xiang,WANG Qin-qin,YANG Zai-dong,ZHU Ying-pu(College of Life Science,Ludong University,Yantai 264025,China) |
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| Abstract: | γ-Polyglutamic acid producing strain yt102 was isolated from soils and taxonomically identified as Bacillus subtilis. A high productive strain of γ-polyglutamic acid was obtained from yt102 with mutagenesis by UV and NTG, and it was genetic ally stable in 10 generations. Fermentation medium composition for the mutant strain was optimized by a single factor and preliminary orthogonal experiments. The average yield of γ-polyglutamic acid reached 28.5 g/L on the optimized medium. |
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| Keywords: | Bacillus subtilis optimization screening |
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