| 红曲多糖液态发酵工艺条件的优化 |
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| 引用本文： | 李霞姣,夏永军,王铁军,许赣荣.红曲多糖液态发酵工艺条件的优化[J].生物加工过程,2009,7(2):13-17. |
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| 作者姓名： | 李霞姣 夏永军 王铁军 许赣荣 |
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| 作者单位： | 江南大学,生物工程学院,无锡,214122 |
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| 摘 要： | 实验研究红曲多糖的液态发酵条件，得出优化后红曲菌ZKOA发酵工艺条件：蔗糖40g/L，酵母粉4．5g／L，KH2PO4·3H2O3．5∥L．MgSO40．4g／L，植物油2mL／L，接种量8％，种龄30h，发酵液起始pH5．0，发酵时间90h，在此条件下，摇瓶和中试发酵罐中的粗多糖质量浓度分别为7．6g／L和7．34g/L。
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| 关 键 词： | 发酵 液态 红曲 优化 多糖 |
Optimization of liquid-fermentation conditions of Monascus polysaccharide |
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| LI Xia-jiao,XIA Yong-jun,WANG Tie-jun,XU Gan-rong.Optimization of liquid-fermentation conditions of Monascus polysaccharide[J].Chinese Journal of Bioprocess Engineering,2009,7(2):13-17. |
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| Authors: | LI Xia-jiao XIA Yong-jun WANG Tie-jun XU Gan-rong |
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| Institution: | ( School of Biotechnology, Jiangnan University, Wuxi 214122, China ) |
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| Abstract: | The liquid-fermentation of Monascus polysaccharide by Monascus sp. ZKOA was investigated. The fermentation conditions were optimized as follows： sucrose 40g/L, yeast extract 4. 5g/L, KH2PQ·3H2O 3.5 g/L, MgSO4 0. 4 g/L, vegetable oil 2 mL/L, seed culture age 30 h, inoculation volume 8% , initial pH of fermentation medium 5.0, and fermentation lasted for 90 h. Under these conditions,the yield of Monascus polysaccharides reached 7.6 g/L and 7.34 g/L in shaking-flask and 20-L fermenter, respectively. |
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| Keywords: | fermentation liquid Monascus optimization polysaccharide |
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