| 连续逆流提取机及其在绞股蓝皂苷提取工艺中的应用 |
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| 引用本文： | 易克传,曾其良,徐淑亮.连续逆流提取机及其在绞股蓝皂苷提取工艺中的应用[J].生物加工过程,2011,9(1):15-18. |
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| 作者姓名： | 易克传 曾其良 徐淑亮 |
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| 作者单位： | 安徽科技学院,工学院,凤阳,233100 |
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| 基金项目： | 安徽高等学校省级自然科学重点研究项目，安徽科技学院稳定人才项目 |
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| 摘 要： | 简述了中草药连续逆流提取机的基本结构和工作原理.以水为溶媒、绞股蓝为原料,进行绞股蓝有效成分的提取试验.以绞股蓝水溶出物和总皂苷为目标物,考察提取温度、液料比和提取时间等因素对提取得率的影响,并通过L9(33)正交实验设计,对提取工艺进行优选.采用中草药连续逆流提取机提取绞股蓝有效成分的最佳工艺条件:提取溶剂温度80 ℃,料液比1:35(g/mL),提取时间50 min;所得提取物得率为33.95%,总皂苷得率为8.9%.
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| 关 键 词： | 逆流提取 绞股蓝 皂苷 |
Continuous countercurrent extraction equipment used in the process of extraction Gypenosides |
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| YI Kechuan,ZENG Qiliang,XU Shuliang.Continuous countercurrent extraction equipment used in the process of extraction Gypenosides[J].Chinese Journal of Bioprocess Engineering,2011,9(1):15-18. |
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| Authors: | YI Kechuan ZENG Qiliang XU Shuliang |
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| Institution: | (College of Technology,Anhui Science and Technology University,Fengyang 233100,China) |
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| Abstract: | The essential structure and the principle of continuous countercurrent extraction equipment on Chinese herbal medicine were introduced.The experiment on extraction effective components of Gynostemma pentaphyllum Makino was studied by water as solvent and G.pentaphyllum Makino as stuff.The key factors influencing extraction yield of wate-solubile matter and saponins,which including extracting temperature,ratio of liquid to solid and extracting time were investigated by using orthogonally experimental design.The results showed that the optimum condition were as follows： liquid to solid ratio 1∶35,temperature 80 ℃,time 50 min.Under these conditions,and the yield of extract was 33.95% and the yield of gypenosides was 8.9%. |
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| Keywords: | countercurrent extraction Gynostemma pentaphyllum saponins |
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