| 一株拜氏梭菌利用甘蔗废糖蜜发酵生产丙酮丁醇 |
| |
| 引用本文： | 孙珊,汪维云,倪晔,王云,宋钢,孙志浩.一株拜氏梭菌利用甘蔗废糖蜜发酵生产丙酮丁醇[J].生物加工过程,2012,10(3):6-11. |
| |
| 作者姓名： | 孙珊 汪维云 倪晔 王云 宋钢 孙志浩 |
| |
| 作者单位： | 1. 安徽农业大学生命科学学院,合肥,230036
2. 江南大学生物工程学院工业生物技术教育部重点实验室,无锡,214122 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划)资助项目 |
| |
| 摘 要： | 以甘蔗废糖蜜作为原料,利用Clostridium beijerinckii DSM 6422菌株进行丙酮丁醇发酵的初步研究.结果表明:采用H2SO4预处理糖蜜,初糖质量浓度60 g/L,(NH4)2SO4 2g/L,CaCO3 10 g/L,温度30℃,pH 5.5～7.0,接种量6％(体积分数),在5L发酵罐中发酵培养96 h,总溶剂产量为16.17 g/L,其中丁醇质量浓度为10.07 g/L,总溶剂产率为30.2％,糖利用率为89.3％.
|
| 关 键 词： | Clostridium beijerinckii 甘蔗废糖蜜 丙酮丁醇 |
Acetone-butanol-ethanol fermentation from cane molasses by Clostridium beijerinckii DSM 6422 |
| |
| SUN Shan
,
WANG Weiyun
,
NI Ye
,
WANG Yun
,
SONG Gang
,
SUN Zhihao.Acetone-butanol-ethanol fermentation from cane molasses by Clostridium beijerinckii DSM 6422[J].Chinese Journal of Bioprocess Engineering,2012,10(3):6-11. |
| |
| Authors: | SUN Shan WANG Weiyun NI Ye WANG Yun SONG Gang SUN Zhihao |
| |
| Institution: | 1.School of Life Science,Anhui Agricultural University,Hefei 230036,China; 2.Key Laboratory of Industrial Biotechnology of the Ministry of Education,School of Biotechnology, Jiangnan University,Wuxi 214122,China) |
| |
| Abstract: | Acetone-butanol-ethanol fermentation using sugar cane molasses by Clostridium beijerinckii DSM 6422 was studied.The molasses were pretreated with H2SO4 and the fermentation conditions including carbon source,nitrogen source,temperature and pH,etc.,were optimized.The results showed that the optimal fermentation conditions was as follows: initial sugar concentration 60 g/L,(NH4)2SO4 2 g/L,CaCO3 10 g/L,inoculation amount 6%(V/V),pH 5.5-7.0,fermented at 30 ℃ for 96 h.Under these conditions,the total solvent,butanol ratio,solvent productivity,and sugar utilization in the 5-L bioreactor were 16.17 g/L,10.07 g/L,30.2%,and 89.3%,respectively. |
| |
| Keywords: | Clostridium beijerinckii sugar cane molasses acetone-butanol-ethanol |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
