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| 地参多糖对实验肿瘤细胞体内外增殖的影响 | |
| 引用本文： | 熊伟,焦扬,罗永会,张翠香,谭德勇,左绍远.地参多糖对实验肿瘤细胞体内外增殖的影响[J].生物加工过程,2012,10(4):64-69. |
| 作者姓名： | 熊伟 焦扬 罗永会 张翠香 谭德勇 左绍远 |
| 作者单位： | 1. 大理学院基础医学院,大理671000;云南大学 生命科学学院,昆明650031 2. 大理学院基础医学院,大理671000;昆明医学院分子与临床医学研究所,昆明650091 3. 大理学院基础医学院,大理,671000 4. 云南大学 生命科学学院,昆明,650031 |
| 基金项目： | 大理学院青年科学基金项目 |
| 摘 要： | 旨在研究地参多糖对小鼠体内S180A肿瘤细胞增殖的影响及体外对人肝癌BEL-7402细胞生长的影响.体内实验采用皮下接种小鼠S180A肿瘤细胞,以生理盐水为阴性对照,环磷酰胺为阳性对照,腹腔给药(ip给药)不同剂量地参多糖7d,称取小鼠瘤质量并计算抑瘤率.体外实验采用四甲基偶氮唑盐(MTT)法测定地参多糖对体外BEL-7402细胞抗肿瘤活性；倒置荧光显微镜观察地参多糖对BEL-7402细胞形态学的影响；流式细胞术检测地参多糖对人肝癌BEL-7402细胞周期的影响.体内实验结果表明:随着地参多糖浓度的增加,体内对小鼠S180A肿瘤的抑制率逐渐提高,最高抑瘤率可达41.29％ (P＜0.01).体外实验结果表明:随着地参多糖浓度和培养时间的增加,体外培养的BEL-7402细胞存活率逐渐降低,抑制率逐渐增加,细胞出现明显的形态学改变,且能诱导细胞凋亡；流式细胞术证实地参多糖使体外培养的BEL-7402细胞发生G0/G1期阻滞.体内外实验均证实地参多糖具有抗肿瘤作用,值得进一步开发利用. |
| 关 键 词： | 多糖 地参 S180A细胞 BEL-7402细胞 细胞增殖 |
Effects of polysaccharide from Lycopus lucidus Turcz. on proliferation of tumor cells in vitro and in vivo |
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| XIONG Wei , JIAO Yang , LUO Yonghui , ZHANG Cuixiang , TAN Deyong , ZUO Shaoyuan.Effects of polysaccharide from Lycopus lucidus Turcz. on proliferation of tumor cells in vitro and in vivo[J].Chinese Journal of Bioprocess Engineering,2012,10(4):64-69. | |
| Authors: | XIONG Wei JIAO Yang LUO Yonghui ZHANG Cuixiang TAN Deyong ZUO Shaoyuan |
| Institution: | 1(1.College of Basic Medicine,Dali University,Dali 671000,China; 2.College of Life Science,Yunnan University,Kunming 650031,China; 3.Institute of Molecular and Clinical Medicine,Kunming Medical University,Kunming 650091,China) |
| Abstract: | The effects of polysaccharide from L.lucidus Turcz.on the preliferation of S180A in vitro and BEL-7402 cells in vivo were investigated.The antiproliferation activities of polysaccharide from L.lucidus Turcz.on BEL-7402 cells were evaluated by MTT assay.The morphologic changes of BEL-7402 cells were observed under fluorescent microscope.Cells division cycle was measured by flow cytometry.The inhibitory rate of polysaccharide against sarcoma 180A in vivo reached 41.29%(P<0.01),the inhibitory rate of BEL-7402 cells increased as the survived rate declined;Polysaccharide increased the number of cells in phase G1,inhibited sarcoma 180A in vivo.It may probably be attributed to the increase of the number of BEL-7402 cells in phase G1 and inhibit cell growth in vivo. |
| Keywords: | polysaccharide L lucidus Turcz S180A cells BEL-7402 cells proliferation |
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