| 离子交换层析纯化透明质酸 |
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| 引用本文： | 刘骥翔,叶华,苏海佳,谭天伟.离子交换层析纯化透明质酸[J].生物加工过程,2009,7(4):32-35. |
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| 作者姓名： | 刘骥翔 叶华 苏海佳 谭天伟 |
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| 作者单位： | 北京化工大学,生命科学与技术学院,北京,100029 |
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| 基金项目： | 国家自然科学基金资助项目，国家高技术研究发展计划(863计划)资助项目 |
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| 摘 要： | 考察6种离子交换树脂的静态吸附解析效果,选出201＊7阴离子交换树脂填柱,确定洗脱流速为0.6mL/min,40mL0.3mol/LNaCl和50mL0.5mol/L NaCl双浓度洗脱,实现透明质酸和杂蛋白的分离。制得透明质酸产品蛋白含量为0.057%,葡萄糖醛酸含量为43%,平均相对分子质量大于1.1×10^6,收率为54%,符合医用级透明质酸行业标准的要求。
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| 关 键 词： | 离子交换层析 纯化 透明质酸 |
Purification of hyaluronic acid by ion exchange chromatography |
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| LIU Ji-xiang,YE Hua,SU Hai-jia,TAN Tian-wei.Purification of hyaluronic acid by ion exchange chromatography[J].Chinese Journal of Bioprocess Engineering,2009,7(4):32-35. |
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| Authors: | LIU Ji-xiang YE Hua SU Hai-jia TAN Tian-wei |
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| Institution: | ( College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China) |
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| Abstract: | A chromatography method was developed to purify hyaluronic acid (HA) of medical usage.Strong acidic cation exchange resin (brand 201*7) was selected for the separation.The optimal elute rate was 0.6 mL/min.The optimal concentrations of eluents were 0.3 mol/L NaCl,and 0.5 moL/L NaCl with the optimal elute volumes of 40 mL and 50 mL,respectively.The protein content of the purified HA was 0.057% and the molecular weight was more than 1.1×10~6.The content of glucuronic acid was 43%.The yield of HA reached 54%.The purified HA reached the industry standard of medical HA. |
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| Keywords: | ion exchange purification hyaluronic acid |
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