| 一种新型亚酞菁荧光探针的合成、表征及动物活体成像研究 |
| |
| 引用本文： | 张泱泱,申剑冰,姚成.一种新型亚酞菁荧光探针的合成、表征及动物活体成像研究[J].生物加工过程,2014(2):70-76. |
| |
| 作者姓名： | 张泱泱 申剑冰 姚成 |
| |
| 作者单位： | 南京工业大学 理学院,南京210009 |
| |
| 基金项目： | 江苏省高校研究生科研创新计划（2012CXZZ12-0444）；南京工业大学材料化学工程国家重点实验室开放课题 |
| |
| 摘 要： | 以4-氨基邻苯二腈、无水ZnCl2、叶酸等为原料,经固相合成法制备了三取代氨基亚酞菁锌(NH2-Sub-PcZn),进一步通过酰胺反应合成了叶酸修饰的三取代氨基亚酞菁锌(FA-Pc)。采用MTT方法评价了NH2-Sub-PcZn、FA-Pc以及四取代氨基酞菁锌(NH2-Pc-Zn)对Hela细胞的毒性,毒性试验表明,加入2 mg/mL FA-Pc,Hela细胞的抑制率超过60%;同时研究了FA-Pc的活体成像特性,近红外荧光成像结果表明,FA-Pc定向聚集在裸鼠的肿瘤肝肾部位且可以稳定12 h。因此说明FA-Pc可用于活体肝癌的选择性成像。
|
| 关 键 词： | 癌细胞 叶酸 亚酞菁 |
Synthesis,characterization and animal whole-body fluorescence imaging application of a new sub-phthalocyanine fluorescent probe |
| |
| ZHANG Yangyang,SHEN Jianbin,YAO Cheng.Synthesis,characterization and animal whole-body fluorescence imaging application of a new sub-phthalocyanine fluorescent probe[J].Chinese Journal of Bioprocess Engineering,2014(2):70-76. |
| |
| Authors: | ZHANG Yangyang SHEN Jianbin YAO Cheng |
| |
| Institution: | ZHANG Yangyang, SHEN Jianbin, YAO Cheng, ZHANG Yangyang, SHEN Jianbin, YAO Cheng |
| |
| Abstract: | Zine amino-sub-phthalocyanine （ NH2-Snb-Pc-Zn） was prepared by solid-state reaction of 4-aminophthalonitrile,anhydrous zinc chloride, folic acid, amidation of the carboxyl.Folic acid （ FA ） modified Pc （ FA-Pc） complexes were obtained by MTT method to evaluate the toxicity to Hela cells with NH2-Sub-Pc-Zn,FA-Pc and four substituted amino-zinc-phthalocyanine（NH2-Pc-Zn）.Toxicity test results showed that 2 mg/mL of FA-Pc led to the inhibition rate of Hela cells of more than 60%.The FA-Pc in vivo imaging characteristics were studied,near-infrared fluorescence imaging results showed that FA-Pc gathered directional in nude mice’ s tumor hepatorenal areas was stable for 12 h.FA-Pc could be used for whole-body fluorescence imaging. |
| |
| Keywords: | cancer cells folic acid sub-phthalocyanine cancer cells folic acid sub-phthalocyanine |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
