| γ-亚麻酸产生菌Mucor sp．EIM-10的筛选及分子鉴定 |
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| 引用本文： | 于爱群,江贤章,夏晓峰,吴松刚,黄建忠.γ-亚麻酸产生菌Mucor sp．EIM-10的筛选及分子鉴定[J].生物加工过程,2009,7(2):74-78. |
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| 作者姓名： | 于爱群 江贤章 夏晓峰 吴松刚 黄建忠 |
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| 作者单位： | 福建师范大学,工业微生物教育部工程研究中心,生命科学学院,福建省现代发酵技术工程研究中心,福州,350108 |
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| 基金项目： | 国家自然科学基金，福建省科技厅重大项目，福建省发改委重大项目 |
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| 摘 要： | 为了获得高产γ-亚麻酸（γ-linolenic acid，GLA）的菌株，利用苏丹黑染色法筛选获得1株GLA产生菌EIM-10，通过摇瓶培养，其生物量可达11．882g／L，菌丝体油脂含量可达18．86％。气相色谱-质谱（GC—MS）分析表明其γ-亚麻酸质量分数（占总脂肪酸）高达27．68％。为进一步鉴定该菌株，克隆测定了该菌18SrRNA基因序列，并对其进行系统进化树分析，结果表明该菌属于毛霉属，与Mucor racemosus、Mucor plumbeus、Mucor ramosissimus与Mucor circinelloides同属一个分支。
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| 关 键 词： | 分析 γ-亚麻酸 Mucor sp． 进化树 18S rRNA |
Isolation and molecular identification of Mucor sp.EIM-10 accumulating GLA |
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| YU Ai-qun,JIANG Xian-zhang,XIA Xiao-feng,WU Song-gang,HUANG Jian-zhong.Isolation and molecular identification of Mucor sp.EIM-10 accumulating GLA[J].Chinese Journal of Bioprocess Engineering,2009,7(2):74-78. |
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| Authors: | YU Ai-qun JIANG Xian-zhang XIA Xiao-feng WU Song-gang HUANG Jian-zhong |
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| Institution: | ( Engineering Research Center of Industrial Microbiology, Ministry of Education, College of Life Science, Fujian Normal University, Engineering Research Center of Fujian Modern Fermentation Technology, Fuzhou 350108,China) |
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| Abstract: | In order to obtain high yield strain of γ-linolenic acid （GLA） EIM-10 accumulating GLA was isolated by using Sudan black staining. After shaking flask culture,the biomass reached 11. 882 g/L,the oil content of the mycelia was 18.86%. The content of GLA was up 27.68% of the total fatty acids by gas chromatography-mass spectrometry （GC-MS）. The strain was further identified at the molecular level. The 18S rRNA gene of EIM-10 was cloned and sequenced. The phylogenetic tree of the 18S rRNA gene of EIM-10 is analyzed. The result showed that the 18S rRNA belonged to Mucor sp., and has the same branch with Mucor racernosus ,Mucor plumbeus ,Mucor ramosissimus, and Mucor circinelloides. |
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| Keywords: | Mucor sp 18S rRNA |
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