| 油菜花粉总黄酮的微波辅助提取及其抗氧化活性研究 |
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| 引用本文： | 焦士蓉,王玲,赵燕,郭子玉,马灵.油菜花粉总黄酮的微波辅助提取及其抗氧化活性研究[J].生物加工过程,2009,7(6):51-54. |
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| 作者姓名： | 焦士蓉 王玲 赵燕 郭子玉 马灵 |
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| 作者单位： | 1. 西华大学,生物工程学院,成都,610039
2. 西华大学,理化学院,成都,610039 |
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| 摘 要： | 采用微波辅助提取法提取油菜花粉总黄酮，通过单因素和正交试验，得到最佳提取条件：80％的乙醇、1：20的料液比、提取时间140s、微波功率242W、提取3次，总黄酮质量分数为（2．64±0．05）％。同时还研究提取物对羟自由基、DPPH自由基的清除作用，以及对小鼠肝组织脂质过氧化的抑制作用，自由基半清除质量浓度和脂质过氧化丰抑制盾奄浓度分剐为0．115、0．325和0．065mg／mL。
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| 关 键 词： | 油菜花粉 总黄酮 微波辅助 抗氧化活性 |
Microwave-assisted extraction of flavonoids from rape pollen and the antioxidant activity of the extracts |
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| JIAO Shi-rong,WANG Ling,ZHAO Yan,GUO Zi-yu,MA Ling.Microwave-assisted extraction of flavonoids from rape pollen and the antioxidant activity of the extracts[J].Chinese Journal of Bioprocess Engineering,2009,7(6):51-54. |
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| Authors: | JIAO Shi-rong WANG Ling ZHAO Yan GUO Zi-yu MA Ling |
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| Institution: | 1. School of Bioengineering of Xihua University, Chengdu 610039, China; 2. School of Physical and Chemical of Xihua University, Chengdu 610039, China) |
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| Abstract: | The extraction of flavonoids from rape pollen with microwave assisted extraction (MAE) and antioxidant activity of the extracts were investigated. Experimental designs of single factor and orthogonal L_9(3~4) were optimized. The optimized conditions were as follows: m(ethanol)/m(rape pollen)=20, microwave power 242 W, and the extraction 3 times with the microwave for 140 s, ethanol concentration 80%. Under these conditions, the yield of total flavonoids was (2.64±0.05)%. The concentrations of extracts for scavenging 50% radical or inhibitor 50% lipid-peroxidation were 0.115 mg/mL, 0.325 mg/mL and 0.065 mg/mL. |
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| Keywords: | rape pollen total flavonoids microwave assisted antioxidant activity |
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