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| 废弃活性污泥中产聚羟基脂肪酸酯菌株Bacillus sp. PB-3的发酵条件优化 | |
| 引用本文： | 郝大可,李强,韩省,王佳佳,罗琰,宫恺.废弃活性污泥中产聚羟基脂肪酸酯菌株Bacillus sp. PB-3的发酵条件优化[J].生物加工过程,2012,10(5):28-33. |
| 作者姓名： | 郝大可 李强 韩省 王佳佳 罗琰 宫恺 |
| 作者单位： | 1. 山东省医学科学院,济南250062;济南大学山东省医学科学院医学与生命科学学院,济南250022 2. 济南大学山东省医学科学院医学与生命科学学院,济南,250022 3. 济南大学化学化工学院,济南,250022 |
| 基金项目： | 国家自然科学基金资助项目（31100088）;公安部应用创新计划资助项目（2010YYCXSDST054） |
| 摘 要： | 通过尼罗红染色法结合荧光显微镜镜检,从废弃活性污泥中分离得到1株高产聚羟基脂肪酸酯(PHAs)的菌株Bacillus sp.PB-3,经气相色谱法鉴定该菌株胞内产物为聚β-羟基丁酸酯(PHB)。对培养基成分及发酵条件优化后,获得最佳培养方案:12 g/L的葡萄糖为C源,2 g/L的牛肉膏为N源,初始pH 7.5,培养基装液量80 mL,转速为200 r/min,37℃培养48 h,PHB质量分数可达菌体干质量的32.09%,比优化前提高30%。 |
| 关 键 词： | 聚羟基脂肪酸酯 聚羟基丁酸酯 筛选 发酵 |
Optimization of PHAs production by Bacillus sp. PB-3 from waste activated sludge |
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| HAO Dake , LI Qiang , HAN Sheng , WANG Jiajia , LUO Yan , GONG Kai.Optimization of PHAs production by Bacillus sp. PB-3 from waste activated sludge[J].Chinese Journal of Bioprocess Engineering,2012,10(5):28-33. | |
| Authors: | HAO Dake LI Qiang HAN Sheng WANG Jiajia LUO Yan GONG Kai |
| Institution: | 1. Shandong Academy of Medical Sciences, Jinan 250062, China; 2. School f Medicine and Life Sciences, University of Jinan and Shandong Academy of Medical Sciences, Jinan 250022, China; 3. School of Chemistry＂ and Chemical Engineering, University of Jinan, Jinan 250022, China) |
| Abstract: | A high-yield strain of PHAs, Bacillus sp. PB-3 was isolated from waste activated sludge by Nile red staining and fluorescence microscopy screening. Gas chromatography analysis indicated that the intracellular production of the strain was polyhydroxybutyrate （ PHB）. Furthermore, medium composition and fermentation factors were investigated and optimized. The optimal carbon and nitrogen sources were glucose （12 g/L） and beef paste （2 g/L）, respectively. The optimal cultivation conditions were pH 7.5, 37 ℃ for 48 h, and 80 mL medium at an agitation rate of 200 r/min. Under the fermentation conditions, the yield of PHB was raised to 32.09% （w/w） 30% higher than that before optimized |
| Keywords: | PHAs PHB screen fermentation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |