| 超声波辅助抽提法在孔雀石绿分子印迹固相萃取中的应用 |
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| 引用本文： | 吕翠翠,袁敏,石服鑫,曹慧,徐斐,于劲松.超声波辅助抽提法在孔雀石绿分子印迹固相萃取中的应用[J].生物加工过程,2014(6):73-77. |
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| 作者姓名： | 吕翠翠 袁敏 石服鑫 曹慧 徐斐 于劲松 |
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| 作者单位： | 上海理工大学 医疗器械与食品学院,上海,200093 |
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| 摘 要： | 采用沉淀聚合法制备孔雀石绿分子印迹聚合物(MG-MIPs),以洗脱效率及吸附量为指标,考察超声波辅助抽提法对MIPs中MG洗脱效果及吸附性能的影响,通过扫描电镜观察MIPs的表面形态,并对其吸附性能进行研究。结果表明:模板分子MG在超声30 min、超声10次、料液比m(MG-MIPs)∶V(甲醇-乙酸溶液)为1∶10(g/m L)、温度为25℃、超声功率为270 W的条件下,洗脱效果最好,MIPs在固相萃取柱中的吸附效率较高,达到198μg/g。
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| 关 键 词： | 分子印迹 固相萃取 模板洗脱 超声波辅助抽提 孔雀石绿 |
Application of the ultrasound-assisted extraction in molecularly imprinted solid phase extraction of malachite green |
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| LYU Cuicui,YUAN Min,SHI Fuxin,CAO Hui,XU Fei,YU Jinsong.Application of the ultrasound-assisted extraction in molecularly imprinted solid phase extraction of malachite green[J].Chinese Journal of Bioprocess Engineering,2014(6):73-77. |
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| Authors: | LYU Cuicui YUAN Min SHI Fuxin CAO Hui XU Fei YU Jinsong |
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| Institution: | (School of Medical Instrument and Food Engineering,University of Shanghai for Science andTechnology,Shanghai 200093,China) |
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| Abstract: | Malachite green（ MG）-molecularly imprinted polymers（ MIPs） were prepared via precipitation polymerization-Using elution efficiency and adsorption of malachite green as indexes, we studied the effects of ultrasonic-assisted extraction on the elution of malachite green and adsorptive property of molecularly imprinted polymers were studied-Surface structure of molecularly imprinted polymers was observed by scanning electron microscope （ SEM ） -Adsorption properties of molecularly imprinted polymers were studied-Results show that the optimum technical parameters for elution efficiency were 30 mins,10 times,the ratio of MG-MIPs to methanol-acetic acid solvent=1/10（g/mL）,25 ℃ and 270 W-The adsorption properties of molecularly imprinted polymers in solid phase extraction column attained 198 μg/g. |
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| Keywords: | molecular imprinting solid phase extraction template removal ultrasound-assisted extraction malachite green |
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